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Plant RNAi and Crop Improvement

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Molecular Techniques in Crop Improvement

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Abstract

RNA interference (RNAi) is a sequence-specific gene silencing technology that is mediated by small interfering RNAs (siRNAs) as a guide of RNA cleavage, translational inhibition and DNA methylation. Most plant RNAi vectors produce hairpin RNAs that are immediately processed into double-stranded siRNAs. RNAi technology has been applied in the genetic engineering of important plant metabolites including starches, oils and storage proteins. RNAi is also useful for preventing plants from viral infection. Since siRNAs (or their precursor, double-stranded RNAs) can be transferred from plants to plant-feeding pests, RNAi has been used to engineer plants resistant to nematodes and insects. In this chapter, several RNAi applications concerning crop improvement are introduced.

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Authors and Affiliations

  1. Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Yokohama, 244-0813, Japan

    Masayuki Isshiki

  2. Graduate School of Horticulture, Chiba University, 648 Matsudo, Chiba, 271-8501, Japan

    Hiroaki Kodama

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  1. Masayuki Isshiki
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  2. Hiroaki Kodama
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Correspondence to Hiroaki Kodama .

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Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Institute (IRRI), Social Sciences Division, International Rice Research, Metro Manila, Philippines

    D.S. Brar

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Isshiki, M., Kodama, H. (2010). Plant RNAi and Crop Improvement. In: Jain, S., Brar, D. (eds) Molecular Techniques in Crop Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2967-6_28

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