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Promoter Trapping in Plants Using T-DNA Mutagenesis

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Molecular Techniques in Crop Improvement

Abstract

Promoter is a DNA fragment with crucial cis-acting signature sequences governing the transcription of an adjoining gene. The promoter elements govern the level of expression of the associated gene, determine the responsiveness of the gene to physical and/or chemical stimuli and decide whether the associated gene would constitutively express or would exhibit tissue or developmental stage specific expression. Depending upon the signal sequences present in the promoter element the expression of the associated gene would respond to one or to a combination of more than one such signal. Promoter element is thus the key component for regulated expression of introduced genes in transgenic plants. A range of promoter elements is being identified and exploited for need-based tailoring of transgenes both for basic and applied purposes. A variety of approaches are employed to isolate promoter elements from plants, amongst them transferred DNA (T-DNA) insertion mutagenesis has been extensively used in identification and cloning of genes, promoters, enhancers and other regulatory sequences. In this chapter, we discuss the T-DNA based approach for identification and isolation of promoter elements from plants. We also highlight the impact of T-DNA tagging strategy in functional genomics studies and discuss some recent developments in the field of cloning, characterization and identification of promoter elements in Arabidopsis thaliana. Finally, we summarize some of the commonly employed approaches for characterization and analysis of plant promoters.

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Acknowledgements

We gratefully acknowledge the financial assistance provided under the NATP, CGP Grant (CGPII/253) for promoter trapping research in Arabidopsis in our laboratory. The study leave grant of IINRG (ICAR) to DS is gratefully acknowledged.

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  1. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, New Delhi, 110012, India

    R. Srinivasan & Dipnarayan Saha

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  1. R. Srinivasan
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Correspondence to R. Srinivasan .

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  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Institute (IRRI), Social Sciences Division, International Rice Research, Metro Manila, Philippines

    D.S. Brar

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Srinivasan, R., Saha, D. (2010). Promoter Trapping in Plants Using T-DNA Mutagenesis. In: Jain, S., Brar, D. (eds) Molecular Techniques in Crop Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2967-6_23

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