Abstract
Microsatellites (or short tandem repeats) offer unprecedented discriminatory power and many other advantages compared to other strain typing methods. They have shown to be excellent tools for discriminating between Aspergillus fumigatus isolates from various origins. Microsatellites are easily amplified by PCR using high stringency conditions. Amplified products are separated according to size using high-resolution equipment such as a DNA sequencer. If properly performed, microsatellite data are straightforward to analyze. However, under certain experimental conditions, well-known PCR artifacts and equipment related issues may complicate interpretation of microsatellite based data and could lead to wrong interpretation of the data. This chapter provides the necessary technical background that needs to be taken into consideration in order to adequately reproduce and interpret a microsatellite based typing assay for A. fumigatus.
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Klaassen, C.H. (2009). Molecular Typing of Aspergillus fumigatus Isolates. In: Comarú Pasqualotto, A. (eds) Aspergillosis: From Diagnosis to Prevention. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2408-4_12
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DOI: https://doi.org/10.1007/978-90-481-2408-4_12
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