Progress in Grapevine protoplast Technology

  • A.K. Papadakis
  • N. Fontes
  • H. Gerós
  • K.A. Roubelakis-Angelakis

During the past four decades, plant protoplasts–naked plant cells lacking walls- have been used for the development of new scientific knowledge involving cell membrane function, cell structure, synthesis of pharmaceutical products, and toxicological assessments. Furthermore, plant proptoplasts are valuable tools for biotechnological applications, such as somatic hybridization protoplast fusion, and genetic transformation by gene transfer mediated through Agrobacterium and Biolistics. A prerequisite for the use of protoplasts genetic engineering is the ability to regenerate plants from the transformed protoplasts. Plant cells possess the unique property of totipotency, that is the ability of somatic cells or protoplasts from fully differentiated, non-dividing to dedifferentiate, re-enter the cell cycle, and proliferate, eventually regenerating the whole plant. Also, in the past decade, few laboratories have employed protoplast transient expression to dissect the function of cis-elements trans-factors in many essential processes and signalling pathways (Sheen 2001).


Somatic Embryogenesis Reactive Oxygen Species Level Reactive Oxygen Species Accumulation Grape Berry Protoplast Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • A.K. Papadakis
    • 1
  • N. Fontes
    • 2
  • H. Gerós
    • 2
  • K.A. Roubelakis-Angelakis
    • 1
  1. 1.Department of BiologyUniversity of CreteGreece
  2. 2.Department of BiologyUniversity of MinhoCampus de GualtarPortugal

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