Abstract
This chapter introduces the basic principles used in applying isotope effects to studies of the kinetics and mechanisms of enzyme catalyzed reactions. Following the introduction of algebraic equations typically used for kinetic analysis of enzyme reactions and a brief discussion of aqueous solvent isotope effects (because enzyme reactions universally occur in aqueous solutions), practical examples illustrating methods and techniques for studying enzyme isotope effects are presented. Finally, computer modeling of enzyme catalysis is briefly discussed.
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Reading List
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Wolfsberg, M., Van Hook, W.A., Paneth, P. (2009). Enzymes; Aqueous Solvent IE’S. In: Isotope Effects. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2265-3_11
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DOI: https://doi.org/10.1007/978-90-481-2265-3_11
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