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Cell Line Development pp 33–52Cite as

Expression Engineering – The IE2 Promoter/Enhancer from Mouse CMV

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Part of the book series: Cell Engineering ((CEEN,volume 6))

Abstract

Cell engineering aims at changing the gene expression program of a specific host cell line for instance by molecular techniques. A widely used strategy is the overexpression of artificially introduced foreign genes. This is achieved by combining the DNA encoding the respective protein of interest with constitutive or regulated promoter sequences, and transfecting the host cell with such recombinant vectors. This approach is taken in a majority of engineering strategies, and a still growing toolbox of different expression governing sequences is becoming available. Some fine examples for such sequences, acting on different aspects of cell engineering, are reviewed throughout this book. Here we introduce the mouse CMV IE2 promoter/enhancer sequence as a new and extremely powerful tool for overexpression of genes of interest. This promoter/enhancer sequence withstands the comparison with established promoter/enhancer combinations commonly used for cell engineering, and often exceeds their performance. We highlight the usefulness of mouse CMV IE2 sequences for the expression of recombinant monoclonal antibodies in connection with the ‘related’ mouse CMV IE1 promoter/enhancer region. This strategy allows us to achieve high expression levels for the production of therapeutic proteins in serum-free mammalian cell culture.

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Acknowledgements

We would like to thank the members of the Merck Serono Biotechnology Center Cell Sciences group for their constant help with experiments and support for generating the reported data. Our special thanks go to the Process Development Analytics group. Christine Power and Mark Ibberson provided essential information on expressed CHO cell sequences.

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  1. Merck Serono Research, Protein and Cell Sciences, Merck Serono Biotechnology Center, 1809, Fenil-sur-Corsier, Switzerland

    Markus O. Imhof, Philippe Chatellard, Michel Kobr, Renata Pankiewicz, Valérie Duverger, Léonard Bagnoud, Christophe Sauvage & Christine Mossu

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  1. Markus O. Imhof
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  2. Philippe Chatellard
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  3. Michel Kobr
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  8. Christine Mossu
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Correspondence to Markus O. Imhof .

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  1. Dept. Chemical & Biochemical, University College, Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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Imhof, M.O. et al. (2009). Expression Engineering – The IE2 Promoter/Enhancer from Mouse CMV. In: Al-Rubeai, M. (eds) Cell Line Development. Cell Engineering, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2245-5_2

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