Next-generation RNA sequencing

  • Alessandro Cellerino
  • Michele Sanguanini
Part of the CRM Series book series (PSNS, volume 17)


The Next-generation RNA sequencing (RNA-seq) is an high-throughput experimental technique (and design) that allows sequencing of cDNA at very high redundancy (depth), in the order of 107 individual sequences (reads) per sample. This technique can provide a quantification-by-sequencing of RNA abundance and analysis of RNA sequence variation from biological samples. The development of high-throughput technologies applied to biology has opened exciting avenues for computational and data-driven biology1.


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Copyright information

© Scuola Normale Superiore Pisa 2018

Authors and Affiliations

  • Alessandro Cellerino
    • 1
  • Michele Sanguanini
    • 2
  1. 1.Scuola Normale SuperiorePisaItaly
  2. 2.Gonville and Caius CollegeUniversity of CambridgeCambridge, CambridgeshireUnited Kingdom

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