Estratto
Rispetto alla matematica o alla fisica, la biologia e la medicina sono scienze empiriche e, poiché non esistono teorie universali che guidano il successo e la progettazione degli esperimenti, nelle scienze biomediche il progresso e le nuove scoperte sono strettamente correlati e dipendenti dall’innovazione tecnologica. La tecnologia del DNA ricombinante, il sequenziamento del DNA, la reazione a catena dell polimerasi (PCR) o la scoperta dei principi per la produzione degli anticorpi monoclonali sono solo alcuni degli esempi di innovazioni tecnologiche che hanno cambiato il modo di fare ricerca biologica negli ultimi 30 anni.
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M.R. Capecchi (1980) High efficiency transformation by direct microinjection of DNA into cultured mammalian cells, Cell 22, pp. 479–488
K.R. Folger, E.A. Wong, G. Wahl, M.R. Capecchi (1982) Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules, Molecular and Cellular Biology 2, pp. 1372–1387
K.R. Thomas, K.R. Folger, M.R. Capecchi (1986) High frequency targeting of genes to specific sites in the mammalian genome, Cell 44, pp. 419–428
J.L Slightom, A.E. Blechl, O. Smithies (1980) Human fetal gγ-and Aγ-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes, Cell 21, pp. 627–638
O. Smithies, R.G. Gregg, S.S. Boggs, M.A. Koralewski, R.S. Kucherlapati (1985) Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination, Nature 317, pp. 230–234
M.J. Evans, M.H. Kaufman (1981) Establishment in culture of pluripotential cells from mouse embryos, Nature 292, pp. 154–156
A. Bradley, M.J. Evans, M.H. Kaufman, E. Robertson (1984) Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines, Nature 309, pp. 255–256
T. Doetschman, R.G. Gregg, N. Maeda, M.l. Hooper, D.W. Melton, S. Thompson, O. Smithies (1987) Targeted correction of a mutant HPRT gene in mouse embryonic stem cells, Nature 330, pp. 576–578
K.R. Thomas, M.R. Capecchi (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells, Cell 51, pp. 503–512
K. Takahashi, K. Tanabe, M. Ohnuki, M. Narita, T. Lchisaka, K. Tomoda, S. Yamanaka (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors, Cell 131, pp. 861–872
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di Massimo, P., Giulia, P. (2009). Perché Mario R. Capecchi, Martin J. Evans e Oliver Smithies hanno vinto il Premio Nobel 2007 per la fisiologia e la medicina?. In: Perché Nobel?. I blu. Springer, Milano. https://doi.org/10.1007/978-88-470-0811-3_8
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DOI: https://doi.org/10.1007/978-88-470-0811-3_8
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