Abstract
The antagonistic potential of indigenous strains of Bacillus, Paenibacillus, and Pseudomonas isolated from potato rhizosphere or soil and soybean plants to manage soil and root diseases of vegetable crops is presented here as a case study. These bacterial strains were also characterised for production or activities of antibiotics, metabolites, volatiles, phytohormones, and lytic enzymes. In agar plate assays, most of the strains showed broad-spectrum antagonistic activity against various selected fungal and oomycete pathogens. Irradiated peat was found to be a suitable carrier material for preparing formulations of these bacteria and delivering them on seed, root, or substrate. In pot experiments, irradiated peat formulations of these bacteria provided control of Pythium damping-off and root rot and Phytophthora blight and root rot of cucumber, Rhizoctonia damping-off of radish, and Fusarium crown and root rot and Fusarium wilt of tomato. Bacterial treatments also resulted in higher fresh weights of plants produced in pathogen-infested substrate. In micro-plot trials, coating of seed potato tubers with irradiated peat formulation of some of these antagonistic bacteria reduced scab severity on daughter tubers and increased tuber weights when treated tubers were planted in a potato soil with a history of common scab disease. These disease suppression and plant-growth promotion activities of various bacterial strains might be related to the production of different antibiotics, secondary metabolites, lytic enzymes, phytohormones, siderophores, and volatiles. The results of these studies indicate that several of the bacterial strains isolated from local sources showed their potential use as biofungicides for protection of seedling damping-off and root rot and tuber diseases caused by various soilborne pathogens.
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References
Abbasi PA (2011) Exploiting and understanding disease suppressing effects of fish emulsion for soil-borne and foliar diseases. Am J Plant Sci Biotechnol 5(Special Issue 2):61–68
Abbasi PA (2013) Establishing suppressive conditions against soilborne potato diseases with low rates of fish emulsion applied serially as a pre-plant soil amendment. Can J Plant Pathol 35:10–19
Abbasi PA, Conn KL, Lazarovits G (2004) Suppression of Rhizoctonia and Pythium damping-off of radish and cucumber seedlings by addition of fish emulsion to peat mix or soil. Can J Plant Pathol 26:177–187
Abbasi PA, Lazarovits G, Conn KL (2008) Enhancing biological control of soilborne plant diseases by organic soil amendments. In: Barka EA, Clément C (eds) Plant-microbe interactions. Research Signpost, Kerala, pp 319–343
Alabouvette C (1999) Fusarium wilt suppressive soils: an example of disease-suppressive soils. Aust Plant Pathol 28:57–64
Anith K, Momol M, Kloepper J, Marios J, Olson S, Jones J (2004) Efficacy of plant growth-promoting rhizobacteria, acibenzolar-S-methyl, and soil amendment for integrated management of bacterial wilt on tomato. Plant Dis 88:669–673
Athukorala SN, Fernando WG, Rashid KY (2009) Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry. Can J Microbiol 55:1021–1032
Bakker PAHM, Ran LX, Pieterse CMJ, van Loon LC (2003) Understanding the involvement of rhizobacteria-mediated induction of systemic resistance in biocontrol of plant diseases. Can J Plant Pathol 25:5–9
Bashan Y, de-Bashan LE, Prabhu SR, Hernandez J-P (2014) Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant Soil 378:1–33
Bernard E, Larkin RP, Tavantzis S, Erich MS, Alyokhin A, Sewell G, Lannan A, Gross SD (2012) Compost, rapeseed rotation, and biocontrol agents significantly impact soil microbial communities in organic and conventional potato production systems. Appl Soil Ecol 52:29–41
Bonanomi G, Antignani V, Capodilupo M, Scala F (2010) Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases. Soil Biol Biochem 42:136–144
Borrego-Benjumea A, Basallote-Ureba MJ, Melero-Vara JM, Abbasi PA (2014) Characterization of Fusarium isolates from asparagus fields in southwestern Ontario and influence of soil organic amendments on Fusarium crown and root rot. Phytopathology 104:403–415
Cook RJ, Baker KF (1983) The nature and practice of biological control of plant pathogens. American Phytopathological Society, St. Paul, 539 pp
Fravel DR (2005) Commercialization and implementation of biocontrol. Annu Rev Phytopathol 43:337–359
Gerhardson B (2002) Biological substitutes for pesticides. Trends Biotechnol 20:338–343
Haas D, Défago G (2005) Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol 3:307–319
Jones KA, Burges HD (1998) Technology of formulation and application. In: Burges HD (ed) Formulation of microbial biopesticides. Kluwer Academic Publishers, Dordrecht/Boston, pp 7–30
Khabbaz SE, Abbasi PA (2014) Isolation, characterization, and formulation of antagonistic bacteria for the management of seedlings damping-off and root rot disease of cucumber. Can J Microbiol 60:25–33
Khabbaz S, Zhang L, Cáceres LA, Sumarah M, Wang A, Abbasi PA (2015) Characterization of antagonistic Bacillus and Pseudomonas strains for biocontrol potential and suppression of damping-off and root rot diseases. Ann Appl Biol 166:456–471
Kim YC, Jung H, Kim KY (2008) An effective biocontrol bioformulation against phytophthora blight of pepper using growth mixtures of combined chitinolytic bacteria under different field conditions. Eur J Plant Pathol 120:373–382
Knowles JW (1976) Microorganisms and cyanide. Bacteriol Rev 40:652–680
Lugtenberg BJJ, Kamilova F (2009) Plant growth promoting rhizobacteria. Annu Rev Microbiol 63:541–556
Mathre DE, Cook RJ, Calla NW (1999) From discovery to use: traversing the world of commercializing biocontrol agents for plant disease control. Plant Dis 83:972–983
Nautiyal CS, Johri JK, Singh HB (2002) Survival of the rhizosphere-competent biocontrol strain Pseudomonas fluorescens NBRI2650 in the soil and phytosphere. Can J Microbiol 48:588–601
Neilands JB (1981) Microbial iron compounds. Annu Rev Biochem 50:715–731
Olubukola OB (2010) Beneficial bacteria of agricultural importance. Biotechnol Lett 32:1559–1570
Oostendorp M, Kunz W, Dietrich B, Staub T (2001) Induced disease resistance in plants by chemicals. Eur J Plant Pathol 107:19–28
Park K, Park J-W, Lee S-W, Balaraju K (2013) Disease suppression and growth promotion in cucumbers induced by integrating PGPR agent Bacillus subtilis strain B4 and chemical elicitor ASM. Crop Prot 54:199–205
Raaijmakers JM, Paulitz TC, Steinberg C, Alabouvette C, Moënne-Loccoz Y (2009) The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321:341–361
Ramamoorthy V, Viswanathan R, Raguchander T, Prakasam V, Samiyappan R (2001) Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Prot 20:1–11
Santoyo G, Orozco-Mosqueda MDC, Govindappa M (2012) Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review. Biocontrol Sci Tech 22:855–972
Trüper HG, dè Clari L (1997) Taxonomic note: necessary correction of specific epithets formed as substantives (nouns) “in apposition”. Int J Syst Bacteriol 47:908–909
Van Veen JA, Van Overbeek LS, Van Elsas JD (1997) Fate and activity of microorganisms introduced into soil. Microbiol Mol Biol Rev 61:121–135
Vidhyasekaran P, Muthamilan M (1995) Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis 79:782–786
Vidhyasekaran P, Rabindran R, Muthamilan M, Nayar K, Rajappan K, Subramanian N, Vasumathi K (1997) Development of a powder formulation of Pseudomonas fluorescens for control of rice blast. Plant Pathol 46:291–297
Weller DM (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Weller DM, Raaijmakers JM, Gardener BBM, Thomashow LS (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu Rev Phytopathol 40:309–348
Zhang L, Khabbaz SE, Wang A, Li H, Abbasi PA (2015) Detection and characterization of broad-spectrum anti-pathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato. J Appl Microbiol 118:685–703
Acknowledgements
Technical assistance was provided by Brian Weselowski, Bruce McPherson, and Igor Lalin. The funding for the research work reported in this book chapter was provided by Agriculture and Agri-Food Canada.
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Abbasi, P.A., Khabbaz, S.E., Zhang, L. (2016). Bioformulations of Novel Indigenous Rhizobacterial Strains for Managing Soilborne Pathogens. In: Arora, N., Mehnaz, S., Balestrini, R. (eds) Bioformulations: for Sustainable Agriculture. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2779-3_8
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DOI: https://doi.org/10.1007/978-81-322-2779-3_8
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