Microheterogeneity of Proteins: Role in Diseases

  • Waliza Ansar
  • Shyamasree Ghosh


Protein posttranslational modifications largely influence their biological functions. The functions included effect on receptor binding, metabolism of the protein, tissue uptake, degradation, excretion, and protein–protein interactions. Modifications of proteins at and after translation also called as posttranslational modifications or PTM occur at a normal physiological concentration of the protein. However, in some diseases, modifications highly specific to the disease also are known to occur. Thus, disease-specific posttranslational modifications find importance as diagnostic markers in clinical medicine. Different approaches, most of which are analytical to study the posttranslational modifications in protein together their interactions, and their complex structure and function are illustrated by various researchers in immunology, biochemistry, biophysics, and clinical medicine and in proteomics. Immuno assay based affinity purification of protein by HPLC and chromatographic methods, and characterization of proteins by lectin binding assays, biophysical methods like MS or mass spectrometry, and surface-enhanced laser desorption/ionization method find importance as diagnostic techniques in clinical proteomic studies, in the detection of the disease-specific protein modification.


Microheterogeneity Disease Posttranslational modifications Clinical proteomics 


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Copyright information

© Springer India 2016

Authors and Affiliations

  • Waliza Ansar
    • 1
  • Shyamasree Ghosh
    • 2
  1. 1.Department of ZoologyBehala CollegeKolkataIndia
  2. 2.National Institute of Science EducationSchool of Biological SciencesBhubaneswarIndia

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