Introduction

Abstract

Hydrogen has enormous potential to serve as a non-polluting fuel and is often hailed as a source of unlimited clean power. Hydrogen production is light-reliant on, since the [FeFe] hydrogenases are coupled to the photosynthetic electron transport chain via ferredoxin. Algal [FeFe] hydrogenases are one of the most active biocatalysts for the evolution of hydrogen. Hydrogenases catalyze a simple reaction, specifically the reversible reduction of protons to molecular hydrogen. The finding of this class of enzymes was made in the 1930s. Stuart and Gaffron were the principal to reveal the direct acquaintances amongst hydrogen progression and photosynthesis (Stuart and Gaffron 1972) and in the late 1990s, Melis and co-workers conventional sulfur deprivation for semi-continuous, photobiological hydrogen production in C. reinhardtii (Melis et al. 2001). C. reinhardtii HydA1 obtains electrons from reducing finale in photosynthetic electron transfer chain. The PetF aircrafts electrons from PSI (photosystem I) to HydA1 which diminishes protons to hydrogen (Melis and Happe 2001). The hydrogenase strives with diverse electron basins, in precise ferredoxin-NADP-oxidoreductase as an edge with the Calvin cycle (Paulette et al. 2003; Knaff 1996; Hemschemeier et al. 2008).