Abstract
Tissues were minced with sharp scissors and then thawed and homogenized with the aid of York’s homogenizer fitted with Teflon plunger in a proportion of 1:10(w/v) ice-cold phosphate buffer (50 mM; PH 7.4). Aliquots of homogenates were used for the determination of malondialdehyde (MDA), hydroperoxides, reduced glutathione (GSH), and total sulfhydryl (TSH) levels. The homogenates were centrifuged for 60 min at 10,000× g at 4 °C, and determination of superoxide dismutase (SOD) and catalase (CAT) activities were carried out with the resultant supernatant. Protein concentration was determined according to the method of Lowry et al. (1951) using purified bovine serum albumin as standard.
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Nabi, S. (2014). Biochemical Estimation Techniques. In: Toxic Effects of Mercury. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1922-4_10
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DOI: https://doi.org/10.1007/978-81-322-1922-4_10
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