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Diversity of Arbuscular Mycorrhizal Fungi in Field and Trap Cultures from Rhizosphere Soils of Flemingia vestita Benth. ex Baker

  • L. S. SongachanEmail author
  • H. Kayang
Chapter

Abstract

Diversity of arbuscular mycorrhizal fungi (AMF) was investigated from natural and cultivated sites of Flemingia vestita (Fabaceae). Sampling was done at monthly intervals for two crop cycles. Root samples were evaluated for AMF colonization in the form of arbuscules, vesicles and hyphae. AMF colonization and spore density was higher in natural site as compared to cultivated site. A total of 68 AMF species were isolated belonging to six genera: Acaulospora, Ambispora, Gigaspora, Glomus, Pacispora and Scutellospora. Trap cultures of AMF were also established with four different host plants (Oryza sativa L., Paspalum notatum Flüggé, Trifolium ripens L. and Zea mays L.) using rhizosphere soils from natural and cultivated sites. A total of 33 AMF species were isolated from trap cultures, out of which three species were not detected in F. vestita rhizosphere soil. Thus, trap cultures allow development and sporulation of AMF species which otherwise were not recovered from field soil.

Keywords

Arbuscular mycorrhizal fungi Flemingia vestita Species 

Notes

Acknowledgment

The authors are thankful to Head, Department of Botany, North Eastern Hill University for providing laboratory facilities. The first author is also grateful to the Rajiv Gandhi National Fellowship, New Delhi, for financial support in the form of research fellowship.

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Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Microbial Ecology Laboratory, Department of BotanyNorth Eastern Hill UniversityShillongIndia

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