Skip to main content

Table 17.3 Culture media used at different stages of Banana micropropagation at the National Research Centre of Banana, India (after Singh et al. 2011)

From: Micropropagation

Ingredients Amount (mg L−1)
Initiation Shoot
multiplication
Rooting
KNO3 1900 1,900 1,900
NH4NO3 1,650 1,650 1,650
CaCl2.2H2O 440 440 440
MgSO4.7H2O 370 370 370
KH2PO4 170 170 170
Fe-EDTA 36.7 36.7 36.7
MnSO4.2H2O 22.3 22.3 22.3
ZnSO4.7H2O 8.6 8.6 8.6
H3BO3 6.2 6.2 6.2
Na2MoO4.2H2O 0.25 0.25 0.25
CoCl2.6H2O 0.025 0.025 0.025
CuSO4.5H2O 0.025 0.025 0.025
L-ascorbic acid 10 10
Glycine 2 2 2
Nicotinic acid 0.5 0.5 0.5
Pyridoxine. HCl 0.5 0.5 0.5
Activated charcoal 2.5
BAP 4 4
IAA 1 1
IBA 1
NAA 2
Sucrose 3 % 3 % 3 %
Agar 0.75 % 0.75 %
Phytagel 0.2 %
pH 5.8 5.8 5.8