• Sant Saran BhojwaniEmail author
  • Prem Kumar Dantu


Clonal propagation of plants, which refers to multiplication of genetically identical individuals by asexual methods of regeneration from somatic tissues or organs, is a common practice in horticulture and forestry to preserve the desirable characters of selected genotypes or varieties in the progeny. Traditionally, it is achieved by cuttings, layering, splitting, grafting and so on. However, for many plants, especially the orchids and tree species, these methods are either very difficult or painfully slow. Since mid 1960s tissue culture has become an established industrial technology being widely used the world over to multiply orchids, other ornamentals, and fruit and forest tree species. In vitro clonal propagation of plants is popularly called micropropagation because of the miniaturization of the process. The major advantages of micropropagation over the conventional methods of clonal propagation are that it is much faster and goes on round the year, protected from pests, pathogens and vagaries of nature. This chapter describes the techniques of micropropagation, the factors that affect the various stages of micropropagation, and commercial aspects of micropropgation and discusses some of the common problems associated with it. The chapter is annexed with protocols and media composition for the micropropagation of selected crops.


Somatic Embryogenesis Clonal Propagation Shoot Multiplication Thin Cell Layer Orchid Seed 
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Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational Institute (Deemed University)AgraIndia

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