Abstract
The monoclonal antibody HNK-1 was raised against the membrane fraction of the human HSB-2 T-cell line. The antigen was originally found to be a marker of human natural killer (HNK) cells and is called as CD57 in immunology. After that, it was found that the HNK-1 carbohydrate epitope is highly expressed in the nervous system, especially on a series of cell adhesion molecules, including neural cell adhesion molecule (NCAM), myelin-associated glycoprotein (MAG), L1, P0, telencephalin, and also some glycolipids. The structure of the HNK-1 epitope was demonstrated to comprise the sulfated trisaccharide HSO3-3GlcAβ1-3Galβ1-4GlcNAc, which is shared by glycolipids and glycoproteins. The expression of the HNK-1 carbohydrate epitope is spatially and temporally regulated during the development of the nervous system, and it functions in cell adhesion, migration, neurite outgrowth and synaptic plasticity (Yamamoto et al. 2002).
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Kakuda S, Shiba T, Ishiguro M, Tagawa H, Oka S, Kajihara Y, Kawasaki T, Wakatsuki S, Kato R (2004) Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1. J Biol Chem 279: 22693–22703
Kakuda S, Sato Y, Tonoyama Y, Oka S, Kawasaki T (2005) Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate. Glycobiology 15:203–210
Shiba T, Kakuda S, Ishiguro M, Morita I, Oka S, Kawasaki T, Wakatsuki S, Kato R (2006) Proteins 65:499–508
Yamamoto S, Oka S, Inoue M, Shimuta M, Manabe T, Takahashi H, Miyamoto M, Asano M, Sakagami J, Sudo K, Iwakura Y, Ono K, Kawasaki T (2002) Mice deficient in nervous system-specific carbohydrate epitope HNK-1 exhibit impaired synaptic plasticity and spatial learning. J Biol Chem 277:27227–27231
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Kakuda, S., Kawasaki, T., Oka, S. (2008). Glucuronyltransferases Involved in the HNK-1 Biosynthesis. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77922-3_12
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DOI: https://doi.org/10.1007/978-4-431-77922-3_12
Publisher Name: Springer, Tokyo
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