Abstract
Subsets of 20 soft keratin polypeptides have been identified in animal tissues by two-dimensional gel electrophoresis [1–6]. Differences among sets of keratins detected by immunocytochemistry distinguish types of epithelia or even specific regions of an epithelium [1,7–10]. From morphologic considerations we hypothesized that taste buds were islets of simple epithelium embedded in stratified squamous epithelium. If this hypothesis is correct, the keratins in taste buds ought to be distinctive from those of surrounding cells. We examined several gustatory epithelia with antikeratin antibodies to compare the immunoreactivity of fusiform, perigemmal, and basal gustatory cells and to identify useful cell markers. The present report concludes that antibodies against keratins 8 and 19 may be used as general differentiation markers for taste receptor cells.
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References
Moll R, Franke WW, Schiller D, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured 11. cells. Cell 31: 11–24
Achtstätter T, Moll R, Moore B, Franke WW (1985) Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract: immunofluorescence and gel electrophoretic studies. J Histochem 33: 415–426
Quinlan RA, Schiller DL, Hatzfeld M, Achtstätter T, Moll R, Jorcano JL, Magin TM, Franke WW (1985) Patterns of expression and organization of cytokeratin intermediate filaments. Ann NY Acad Sci 455: 282–306
Bartek J, Bartkova J, Taylor-Papadimitriou J, Rejthar A, Kovarik J, Lukas Z, Vojtesek B (1986) Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J 18: 565–575
O’Guin WM, Schermer A, Lynch M, Sun TT (1990) Differentiation-specific expression of keratin pairs. In: Goldman RD, Steinert PM (eds) Cellular and mole-cular biology of intermediate filaments. Plenum, New York, pp 301–334
Moll R, Lowe A, Laufer J, Franke WW (1992) Cytokeratin 20 in human carcinomas. Am J Pathol 140: 427–447
Franke WW, Schmid E, Schiller DL, Winter S, Jarash ED, Moll R, Denk H, Jackson B, Illmensee K (1982) Differentiation-related patterns of expression of 19. proteins and intermediate-sized filaments in tissues and cultured cells. Cold Spring Harbor Symp Quant Biol 46: 431–453
Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, 20. Woodcock-Mitchell J, Sun TT (1982) Correlation of specific keratins with different types of epithelial 21. differentiation: monoclonal antibody studies. Cell 30: 361–372
Cooper D, Schermer A, Sun TT (1985) Biology of disease classification of human epithelia and their 22. neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab Invest 52: 243–256
Sun TT, Tseng SCG, Huang AJ-W, Cooper D, Schermer A, Lynch MH, Weiss R, Eichner R (1985) Monoclonal antibody studies of mammalian epithelial keratins: a review. Ann NY Acad Sci 445: 307–329
Oakley B, Schafer R (1978) Experimental Neurobiology. University of Michigan Press, Ann Arbor, p 355
Roper SD (1987) The cell biology of vertebrate taste receptors. Annu Rev Neurosci 12: 329–353
Murray RG (1974) The ultrastructure of taste buds. In: Friedmann I (ed) The ultrastructure of sensory organs. Elsevier, New York, pp 1–81
Lane EB (1982) Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol 92: 665–673
Takeda M, Obara N, Suzuki Y (1990) Keratin filaments of epithelial and taste-bud cells in the circumvallate papillae of adult and developing mice. Cell Tissue Res 260: 41–48
Sawaf MH, Ouhayoun JP, Shabana AHM, Forest N (1990) Cytokeratin expression in human tongue epithelium. Am J Anat 189: 155–166
Oakley B (1991) Neuronal-epithelial interaction in mammalian gustatory epithelium. In: Whelan J (ed) Regeneration of vertebrate sensory receptor cells (vol 160 ). Wiley, Chichester, pp 277–287
Gigi-Leitner O, Geiger B (1986) Antigenic interrelationship between the 40-kilodalton cytokeratin polypeptide and desmoplakins. Cell Motil Cytoskel 6: 628–639
Stasiak PC, Purkis PE, Leigh IM, Lane EB (1989) Keratin 19: predicted amino acid sequence and broad tissue distribution suggest it evolved from keratinocyte keratins. J Invest Dermatol 92: 707–716
Simmons K, Fuller SD (1985) Cell surface polarity in epithelia. Annu Rev Cell Biol 1: 243–288
Matlin KS, Caplan MJ (1992) Epithelial cell structure and polarity. In: Seldin DW, Giebish G (eds) The kidney: physiology and pathophysiology ( 2nd ed. ). Raven, New York, pp 447–473
Cummings TA, Kinnamon SC (1992) Apical K+ channels in Necturus taste cells: modulation by intracellular factors and taste stimuli. J Gen Physiol 99: 591–613
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© 1994 Springer Japan
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Oakley, B., Lawton, A., Wong, L., Zhang, C. (1994). Keratin Polypeptides and Taste Buds. In: Kurihara, K., Suzuki, N., Ogawa, H. (eds) Olfaction and Taste XI. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68355-1_5
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DOI: https://doi.org/10.1007/978-4-431-68355-1_5
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68357-5
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