Summary
The stellate cell is a principal producer of extracellular matrix proteins in the liver and plays a major role in the development of liver fibrosis. The molecular basis of cell activation has therefore been intensively analyzed during the past decade. In order to reveal the total gene/protein synthesis in stellate cells, in particular the dynamic change in their level of expression in response to activation, suppression subtractive hybridization and proteomics/genomics analysis were performed. In suppression subtractive hybridization, 11 genes dominantly expressed in activated stellate cells were identified. One of them was cellular prion protein, whose expression was clearly restricted to activated stellate cells also in the in vivo fibrosis model. Over 150 stellate cell genes/proteins were successfully analyzed by proteomics/genomics, and several of them, including novel observations, represent activation-associated change. Thus, molecules associated with stellate cell activation and consequently liver fibrosis may remain undiscovered. Comprehensive understanding of the molecular basis of liver fibrosis is required.
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© 2001 Springer-Verlag Tokyo
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Kawada, N. (2001). Analysis of Proteins and Genes Dominantly Expressed in Stellate Cells of Activated Phenotype: the Molecular Approach to Liver Fibrosis. In: Okita, K. (eds) Liver Cirrhosis. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68343-8_2
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DOI: https://doi.org/10.1007/978-4-431-68343-8_2
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68345-2
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