Summary
Clonal immunoglobulin heavy chain (IgH) gene rearrangement was detected by polymerase chain reaction (PCR) and PCR conjuncting with single-strand conformational polymorphism (PCR-SSCP) in 11 bone marrow transplantation (BMT) patients with acute lymphoblastic leukemia (ALL) for detecting minimal residual disease (MRD). This study suggested that the presence of marker positive cells in complete remission pre-transplantation or persistence in the post-transplantation period is an indication of poor prognosis or long-term disease-free survival. Using clonal IgH gene rearrangement by PCRSSCP to detect MRD pre- and post-transplantation may provide some important information for selecting a suitable time, as well as assessing the prognosis and detecting the effect of in vitro marrow purging for bone marrow transplantation.
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Trainor KJ, Brisco MJ, Wan JH, Neoh S. Grist S, Morley AA. (1991). Gene rearrangement in B-and T-lymphoproliferative disease detected by the polymerase chain reaction. Blood 78: 192–196
Wan JH,Trainor KJ,Brisco MJ, Morle AA. (1990). Monocolonality in B cell lymphoma detected in paraffin was embedded sections using the polymerase chain reaction. J Clin Pathol 43 888–890
Li Yu, Yan Sun, Qi Zhou, Jing-Zhong Liu, Fang-Ding Lou. (1995). Detection of minimal tumour cell in bone marrow of the patients with B-cell lineage neoplasms by PCR-SSCP. Chinese J Hematology 16 (7): 372.
Davis TH,Yockey CE,Balk SP. (1993) Detection of clonal immunoglobulin gene earrangement by polymerase chain reaction amplification and single strand conformational polymorphism analysis. Am J Pathol 142: 1841–1847
Korsmeyer SJ, Arnold A, Bakhshi A. (1983) Immunoglobulin gene rearrangement and cell surface antigen expression in acute lymphocytic leukemias of T cell and B cell precursor origins. J Clin Invest 71: 301–313
Liang R, Chan V, TK, Wong T, Chiu E. (1993) Detection of immunoglobulin gene rearrangement in lympoid malignancies of B-cell lineage by seminested polymerase chain reaction gene amplification. Am J Hematol 43: 24–28
Potter MN, Cross NCP, Van Dongen JJM. (1993) Molecular evidence of minimal residual disease after treatment for leukemia and lymphoma: an updated meeting report and review. Leukemia 7: 1302
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© 1996 Springer-Verlag Tokyo
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Zhou, Q., Yu, L., Lou, FD. (1996). Detection of Minimal Residual Disease by Immunoglobulin Heavy Chain Gene Rearrangement Analysis in Patients with Ball after Bone Marrow Transplantation. In: Ikehara, S., Takaku, F., Good, R.A. (eds) Bone Marrow Transplantation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68320-9_36
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DOI: https://doi.org/10.1007/978-4-431-68320-9_36
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68322-3
Online ISBN: 978-4-431-68320-9
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