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HCV-specific Translation Initiation

  • Conference paper
Viral Hepatitis and Liver Disease

  • 24 Accesses

Abstract

In vitro translation experiments involving reticulocyte and HeLa S3 cell lysates have demonstrated that an internal ribosome entry site (IRES) resides within the 5′ untranslated region (UTR) of hepatitis C virus (HCV) RNA. Furthermore, it has been shown that the function of the IRES typical to group II HCV-RNA is more efficient than IRES typical to group I in a cell-free protein synthesis system from HeLa S3 cells. A number of recombinant HCV-RNAs between efficient and inefficient HCV mRNAs were constructed with regard to the 5′UTR, and examined for their mRNA translation efficiences in a cell-free system from HeLa S3 cells. Most recombinant RNAs showed levels of initiation activity similar to or higher than typical group II HCV, strongly suggesting that efficiency of the translation initiation is generally suppressed in nature. These results suggest that persistent infection of HCV in humans requires the downregulated IRES functions.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Dr. Akio Nomoto

  2. Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Tokyo, 113, Japan

    Dr. Akio Nomoto, Kyoko Tsukiyama-Kohara & Michinori Kohara

Authors
  1. Dr. Akio Nomoto
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  2. Kyoko Tsukiyama-Kohara
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  3. Michinori Kohara
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Editor information

Editors and Affiliations

  1. Japanese Red Cross, Tokyo, Japan

    K. Nishioka M.D. (Chairman of 1993 ISVHLD) (Chairman of 1993 ISVHLD)

  2. Yamanashi Medical College, Yamanashi, Japan

    H. Suzuki (Vice Chairman of 1993 ISVHLD) (Vice Chairman of 1993 ISVHLD)

  3. Institute of Immunology, Tokyo, Japan

    S. Mishiro (Committee Member of 1993 ISVHLD) (Committee Member of 1993 ISVHLD)

  4. Japanese Red Cross Medical Center, Tokyo, Japan

    T. Oda (Honorary Chairman of 1993 ISVHLD) (Honorary Chairman of 1993 ISVHLD)

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© 1994 Springer-Verlag Tokyo

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Nomoto, A., Tsukiyama-Kohara, K., Kohara, M. (1994). HCV-specific Translation Initiation. In: Nishioka, K., Suzuki, H., Mishiro, S., Oda, T. (eds) Viral Hepatitis and Liver Disease. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68255-4_32

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  • DOI: https://doi.org/10.1007/978-4-431-68255-4_32

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68257-8

  • Online ISBN: 978-4-431-68255-4

  • eBook Packages: Springer Book Archive

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