Summary
After maximal vasodilation and KCl or BaCl2 heart arrest (groups D and S, respectively), vessel wall fibronectin was labelled in the in situ frozen myocardium of ten Wistar rats. Capillary and venular dimensions and densities were measured in transverse sections of the inner third of the left ventricular free wall for both groups. The measured capillary diameters and density were, respectively, 6.86 ± 1.73 μm and 1434 ± 233/mm2 in group D, and 4.97 ± 1.12 μm and 1316 ± 400/mm2 in group S. The venular cross- sectional areas were 181 ± 27 μm2 in group S and 415 ± 65 μm2 in group D, while the respective venular densities were 15.4 ± 3.5/mm2 and 17.1 ± 2.9/mm2. These morphometric measurements correspond to an intramyocardial blood volume of 6.0 ml/100 g of left ventricle (LV) in group D and 2.9 ml/100g LV in group S. These results suggest: that (1) even in the group with BaCl2 arrest, there are very few subendocardial capillaries smaller than the critical value for red cell passage, and (2) the systolic-diastolic changes in intramyocardial blood volume may form a significant part of the total vasodilated intramyocardial blood volume.
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References
Spaan JAE (1985) Coronary diastolic pressure-flow relation and zero flow pressure explained on the basis of intramyocardial compliance. Circ Res 56:293–308
Bassingthwaighte JB, Yipintsoi T, Harvey RB (1974) Microvasculature of the dog left ventricular myocardium. Microvasc Res 7:229–249
Potter RF, Groom AC (1983) Capillary diameter and geometry in cardiac and skeletal muscle studied by means of corrosion casts. Microvasc Res 25:68–84
Tillmanns H, Ikeda S, Hansen H, Sarma JSM, Fauvel JM, Bing RJ (1974) Microcirculation in the ventricle of the dog and turtle. Circ Res 34:561–569
Henquell L, LaCelle PL, Honig CR (1976) Capillary diameter in rat heart in situ: Relation to erythrocyte deformability, O2 transport, and transmural O2 gradients. Microvasc Res 12:259–274
Steinhausen M, Tillmanns H, Thederan H (1978) Microcirculation of the epimyo- cardial layer of the heart. A method for in vivo observation of the microcirculation of superficial ventricular myocardium of the heart and capillary flow pattern under normal and hypoxic conditions. Pflügers Arch 378:9–14
Nellis SH, Liedtke AJ (1982) Pressures and dimensions in the terminal vascular bed of the myocardium determined by a new free-motion technique. In: Tillmanns H, Kubier W, Zebe H (eds) Microcirculation of the heart. Springer, New York Berlin Heidelberg Tokyo, pp 61–74
Kotelianski VE, Arsenyeva EL, Bogacheva GT, Chernousov MA, Glukhova MA, Ibrighimov AR, Metsis ML, Petrosyan MN, Rokuin OV (1982) Identification of the species-specific antigenic determinant(s) of human plasma fibronectin by monoclonal antibodies. FEBS Lett 42:199–202
Clarks AJR, Clarks PAA (1983) Capture of spatially homogenous chemical reactions in tissue by freezing. Biophys J 42:25–30
Reynold SRM, Kirsch M, Bing RJ (1958) Functional capillary beds in the beating, KCI-arrested and KCI-arrested-perfused myocardium of the dog. Circ Res 6:600–611
Buss DD, Hyde DM, Stoval MY (1981) Application of stereology to coronary microcirculation. Basic Res Cardiol 76:411–415
Martini J, Honig CR (1969) Direct measuremant of intercapillary distance in beating rat heart in situ under various conditions of O2 supply. Microvasc Res 1:244–256
Duran WN (1982) Myocardial capillary recruitment studied by indicator dilution curves. In: Tillmanns H, Kubier W, Zebe H (eds) Microcirculation of the heart. Springer, New York Berlin Heidelberg Tokyo, pp 109–117
Tomanek RJ, Searls JC, Lachenbruch PA (1982) Quantitative changes in the capillary bed during developing peak and stabilized cardiac hypertrophy in the spontaneously hypertensive rat. Circ Res 51:295–304
Vetterlein F, Hemeling H, Sammler G, Pettio A, Schmidt G (1989) Hypoxia-induced acute changes in capillary and fiber density and capillary red cell distribution in the rat heart. Circ Res 64:742–752
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© 1993 Springer-Verlag Tokyo
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Levy, B.I., Samuel, J.L., Kotelianski, V.E., Marotte, F., Poitevin, P., Chadwick, R.S. (1993). Morphometric Measurement of Subendocardial Vessel Dimensions in Systolic and Diastolic Arrested Rat Heart. In: Maruyama, Y., Kajiya, F., Hoffman, J.I.E., Spaan, J.A.E. (eds) Recent Advances in Coronary Circulation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68249-3_9
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DOI: https://doi.org/10.1007/978-4-431-68249-3_9
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