Abstract
The production by microorganisms of large amounts of proteins which are present only in small amounts in nature is one of the most important subjects of current biotechnology. Many expression systems have been developed in which microorganisms are used as hosts. The amounts of heterologous proteins produced in the cytoplasm of microorganisms (especially Escherichia coli) sometimes reaches 50% of the total cellular protein. However, these proteins often become insoluble and inactive, forming so-called inclusion bodies. Their conversion to their active forms requires additional time- and cost-consuming processes. In contrast, proteins secreted into the medium tend to remain soluble and active. Moreover, since there is no space limit, larger amounts of proteins can be accumulated in the medium than in the cytoplasm or periplasm of the microorganisms. Bacillus subtilis and Saccharomyces cerevisiae have been extensively studied as to the secretion of mammalian proteins in their active forms, but the productivity has been mostly low.
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© 1992 Springer-Verlag Tokyo
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Yamagata, H. (1992). Development of a Bacillus brevis Host-Vector System for Efficient Heterologous Protein Production and Its Application to Protein Engineering. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_4
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DOI: https://doi.org/10.1007/978-4-431-68180-9_4
Publisher Name: Springer, Tokyo
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