Abstract
Cyclodextrin (CD) production has been mainly carried out in a homogeneous enzyme reaction system utilizing soluble liquefied starch (1,2). The shortcomings of above conventional CD production method were low CD yield and difficulties of separation and purification, due to formation of undesirable oligosaccharides (3). Direct production of CD from insoluble raw starch has distinct advantage of formation of high purity of CD without accumulation of undesirable oligosaccharides, which will facilitated purification of CDs (4,5). But raw starch exists as a compact crystalline structure, and the rate and yield of CD formation by cyclodextrin glucanotransferase (CGTase) is too low for industrial application. Therefore the structural modification of raw starch is required to increase the susceptibility to enzyme. In this work, the structre of raw starch was modified by means of extrusion, and the direct synthesis of CD in a heterogeneous enzyme reaction system containing insoluble extruded starch was attempted.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hara K, Hashimoto H (1986) Application of Cyclodextrin, J Jpn Soc Starch Sei 33: 152–161
Horikoshi K, Nakamura M (1979) Process for Production of Cyclodextrin, US Patent. 4, 135, 977
Szejtli J (1988) Cyclodextrin Technology, Kluwer Academic Publishers. Dordrecht Boston London. pp 34–36
Lee YH, Park JS (1989) Evaluation of Operational Conditions and Power Consumption of Bioattritor for Enzymatic Saccharification of Uncooked Starch, Kor J Appl Microbiol Biotechnol 17: 349–357
Lee YH, Park DC (1991) Enzymatic Synthesis of Cyclodextrin in a Heterogeneous Enzyme Reaction System Containing Insoluble Extruded Starch, Kor J Appl Microbiol Biotechnol 19: (in print)
Schmid G (1989) Cyclodextrin Glycosyltransferase Production: Yield Enhancement by Overexpression of Cloned Genes, Tibteck 7: 244–248
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Tokyo
About this paper
Cite this paper
Lee, YH., Park, DC. (1992). Direct Synthesis of Cyclodextrin in a Heterogeneous Enzyme Reaction System Containing Insoluble Extruded Starch. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_33
Download citation
DOI: https://doi.org/10.1007/978-4-431-68180-9_33
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68182-3
Online ISBN: 978-4-431-68180-9
eBook Packages: Springer Book Archive