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A Computerized System for Spot Detection and Analysis of Two-Dimensional Electrophoresis Images

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Computer-Generated Images

Abstract

The 2-dimensional electrophoresis technique is very powerful to separate complex mixtures of proteins. It is estimated that it is possible to separate 19,000 proteins on a single gel (O’Farrel 1975; Anderson 19 77). Because of this large number, the only practical solution for using 2-dimensional electrophoresis is through a computerized system. The main tasks of the system are:

  • image digitization,

  • noise reduction or elimination,

  • spot detection or separation,

  • normalization and standardization.

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References

  • Anderson ML et NG (1977) High resolution two-dimensional separation of human plasma proteins. Proc. Natl. Acad. Sci. USA, 5421–5425.

    Google Scholar 

  • Coultec BP, Anderson NG (1981) The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns. In Clin. Chem., vol. 27, 11, p. 1807–1820.

    Google Scholar 

  • Harrington MG, Merril CR, Goldman G, Xu XH, McFartin DE (1984) Two-dimensional electrophoresis of cerebrospinal fluid proteins in multiple sclerosis and various neurological deseases. In electrophoresis, vol. 5, p. 236–245.

    Article  Google Scholar 

  • Miller MJ et al. (1982) Computer analysis of two-dimensional gels: semi-automatic matching. Clin. Chem., vol. 28, 4, p. 867.

    Google Scholar 

  • O’Farrel PH (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.

    Google Scholar 

  • Wiederkehr F, Vonderschmitt DJ (submitted 1985) Zweidimensionale Elektrophorese des liquor cerebrosbinalis verschiedener neurologischer Patienten.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Centre Universitaire d’informatique, 24, rue du Général-Dufour, 1211, Geneva 4, Switzerland

    R. Appel, M. Funk & C. Pellegrini

  2. Hôpital Cantonal Universitaire, 1211, Geneva 4, Switzerland

    D. Hochstrasser & A. F. Müller

Authors
  1. R. Appel
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  2. M. Funk
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  3. C. Pellegrini
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  4. D. Hochstrasser
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  5. A. F. Müller
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Editor information

Editors and Affiliations

  1. Hautes Etudes Commerciales, Université de Montréal, H3T 1V6, Montréal, Canada

    Nadia Magnenat-Thalmann

  2. Département d’Informatique et de Recherche Opérationnelle, Université de Montréal, Montréal, H3C 3J7, Canada

    Daniel Thalmann

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© 1985 Springer-Verlag Tokyo

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Appel, R., Funk, M., Pellegrini, C., Hochstrasser, D., Müller, A.F. (1985). A Computerized System for Spot Detection and Analysis of Two-Dimensional Electrophoresis Images. In: Magnenat-Thalmann, N., Thalmann, D. (eds) Computer-Generated Images. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68033-8_11

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  • DOI: https://doi.org/10.1007/978-4-431-68033-8_11

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68035-2

  • Online ISBN: 978-4-431-68033-8

  • eBook Packages: Springer Book Archive

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