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Some Phytochemicals and Related Compounds in Vegetables as Potent Inhibitors of Human DNA Topoisomerase II

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Food Factors for Cancer Prevention

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Summary

An established human DNA topoisomerase II inhibitory assay is described, and the screening results for some phytochemicals in vegetables and related compounds that may useful for chemoprevention through food are reported. The assay requires a small amount of sample and is rapid compared to bioassays using cell lines or animals. The topoisomerase II inhibitory assay is useful for screening compounds in vegetables. More than 80 compounds were screened, and 28 compounds were found to be potent inhibitors at the 10 μg/ml level. Many of the phytochemicals commonly found in vegetables are thus useful for chemoprevention.

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References

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Author information

Authors and Affiliations

  1. Division of Foods, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158, Japan

    Makoto Miyahara, Hiroshi Akiyama, Masatake Toyoda & Yukio Saito

  2. Division of Chemistry, Center for Food and Drug Safty at Hadano, 729-5 Ochiai, Hadano City, Kanagawa 257, Japan

    Masaru Kawasaki

  3. Department of Pharmacognosy and Phytochemistry, 1-35-23 Nozawa, Setagaya-ku, Tokyo 154, Japan

    Takao Narui & Toru Okuyama

Authors
  1. Makoto Miyahara
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  2. Masaru Kawasaki
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  3. Hiroshi Akiyama
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  4. Takao Narui
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  5. Masatake Toyoda
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  6. Toru Okuyama
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  7. Yukio Saito
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Editor information

Editors and Affiliations

  1. Department of Food Science and Technology Faculty of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Kakyo-ku, Kyoto, 606-01, Japan

    Hajime Ohigashi Ph.D. (Professor) (Professor)

  2. Department of Applied Biological Sciences Faculty of Agriculture, Nagoya University, Furocho, Chikusa-ku, Nagoya, Aichi, 464-01, Japan

    Toshihiko Osawa Ph.D. (Professor) (Professor)

  3. Lipid Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan

    Junji Terao Ph.D. (Head) (Head)

  4. Nutrition and Epidemiology, Department of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156, Japan

    Shaw Watanabe M.D. (Professor) (Professor)

  5. First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

    Toshikazu Yoshikawa M.D. (Associate Professor) (Associate Professor)

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© 1997 Springer Japan

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Miyahara, M. et al. (1997). Some Phytochemicals and Related Compounds in Vegetables as Potent Inhibitors of Human DNA Topoisomerase II. In: Ohigashi, H., Osawa, T., Terao, J., Watanabe, S., Yoshikawa, T. (eds) Food Factors for Cancer Prevention. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67017-9_37

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  • DOI: https://doi.org/10.1007/978-4-431-67017-9_37

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-67019-3

  • Online ISBN: 978-4-431-67017-9

  • eBook Packages: Springer Book Archive

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