Summary
Our attention has focused on the primary prevention of cancer using functional ingredients of edible plants. Here we report a new antitumor-promoting component, tea aqueous nondialysates (TNDs), and discuss the modes of action. TNDs were prepared from the hot water infusion of green and black tea leaves followed by extraction with CHC13, ethyl acetate, and n-butanol and finally by dialysis. The TNDs had a molecular weight of more than 12000 and consisted of a complex mixture of tannins, hydrolyzable and condensed, containing sugar(s), quinic acid, and polyphenolic compounds such as gallates and catechins. TNDs inhibit the soft agar colony induction by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) without particular cytotoxicity and the morphological changes induced by TPA in the mouse epidermal JB6 cell lines. The chemical structure of TDNs with sugar(s) and polyphenols was required for the activity because the modifications of TNDs by β-glucosidase, polyphenoloxidase, or tannase caused the loss of activity. Reversion of TPA-induced morphological alternations was associated with an increase in the cytoskeletal actin microfilaments and fiber of the extracellular matrix, fibronectin. Therefore the antitumor-promoting effect of TNDs was different from that of green tea catechins.
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Nakamura, Y., Kawase, I., Harada, S., Matsuda, M., Honma, T., Tomita, I. (1997). Antitumor-Promoting Effects of Tea Aqueous Nondialysates in Mouse Epidermal JB6 Cells. In: Ohigashi, H., Osawa, T., Terao, J., Watanabe, S., Yoshikawa, T. (eds) Food Factors for Cancer Prevention. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67017-9_27
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DOI: https://doi.org/10.1007/978-4-431-67017-9_27
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