Abstract
Superior cervical ganglion neurons (SCGNs) of neonatal rats have been known to form cholinergic synapses in culture (O’Lagure et al. 1974). This article illustrates a useful system of mammalian synapse, SCGNs synapse formed in culture, for investigations of molecular mechanisms of neurotransmitter release and their modulation (Mochida et al. 1994ab, 1995, 1996; Mochida 1995). Two features of this preparation are particularly useful for applying electrophysiological studies: 1) the large size of the cell bodies (30–40 µm) of SCGNs and (2) the short diffusion distance from the cell body to release sites at the synaptic terminals. This combination of features makes it possible to introduce molecular probes, such as peptides, proteins, or antibodies into the presynaptic neuron while detecting resultant changes in acetylcholine (ACh) release by measuring postsynaptic electrical responses. With this microinjection approach, we have obtained evidence supporting roles for the interaction of N-type Ca2+channels with synaptic core complex of the synaptic vesicle protein VAMP/synaptobrevin (Trimble et al. 1988) and synaptic membrane proteins syntaxin (Bennett et al. 1992; Inoue et al. 1992; Yoshida et al. 1992) and SNAP-25 (Oyler et al. 1989) in regulating exocytosis at presynaptic terminals.
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Mochida, S. (2000). Studies of Neurotransmitter Release at Cholinergic Synapses Formed Between Sympathetic Neurons in Culture: Role of Ca2+ Channels in Neurotransmitter Release. In: Kuba, K., Higashida, H., Brown, D.A., Yoshioka, T. (eds) Slow Synaptic Responses and Modulation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-66973-9_30
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DOI: https://doi.org/10.1007/978-4-431-66973-9_30
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