Using BIACORE as Pots and Pans

Abstract

A postdoctoral fellow needed to make an antibody affinity column of rather larger size. The antibody is commercially available, but it is expensive. He decided to prepare it by culturing hybridoma offered by a certain laboratory. But this hybridoma did not look very good and its growth rate was poor. Yet by persevering with cloning and culturing, it was eventually revived. So, 10µl of the cultured supernatant was diluted 10-fold and injected onto a Protein A immobilized sensor chip. What we saw was a handsome binding curve (see figure). The culture was then scaled up in 4 1 while monitoring the production amount using BIACORE, and this succeeded in preparing a fairly large amount of antibody