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Surface Fixation of Lumbrokinase via Photochemical Reaction of Azidophenyl Group

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Heart Replacement

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Summary

A new method for the immobilization of lumbrokinase (LK) on polymer was developed. The method is based on the photochemical reaction of the azidophenyl group, which produces a highly reactive phenylnitrene upon ultraviolet (UV) light irradiation. First, an aminated polymer, partially derivatized with a photoreactive phenyl azido group in its side chains, was coated onto a polyurethane (PU) surface. Subsequently, the surface was exposed to UV light. To induce the carboxyl group on the UV-light-treated surface, poly(methyl vinyl ether-co-maleic acid) (MAcMEC) was reacted. LK was immobilized on the PU surface using l-ethyl-3-(3-dimethylaminopropyl)carbo-diimide (EDC) in aqueous solvent. Electron spectroscopy for chemical analysis (ESCA) and water contact angle measurement before and after sequential surface reactions revealed that LK was immobilized successfully. The LK immobilized on the PU surface had fibrinolytic activity. In conclusion, LK immobilization via this photochemical reaction will be useful in improving the blood compatibility of blood-contacting polymer.

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References

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Author information

Authors and Affiliations

  1. Institute of Medical and Biomedical Engineering, Seoul National University Hospital, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea

    Hyun Jung Kim, Jongwon Kim & Byoung Goo Min

  2. Biomedlab Company, Dongsung Bldg., 1-49 Dongsung-Dong, Jongno-Gu, Seoul, 110-510, Korea

    Hyun Jung Kim & Jongwon Kim

  3. National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565, Japan

    Yasuhide Nakayama & Takehisa Matsuda

Authors
  1. Hyun Jung Kim
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  2. Yasuhide Nakayama
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  3. Jongwon Kim
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  4. Takehisa Matsuda
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  5. Byoung Goo Min
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Editor information

Editors and Affiliations

  1. Shonan Center, Terumo Corporation, 1500 Inokuchi, Nakai-machi, Ashigarakami-gun, Kanagawa, 259-01, Japan

    Tetsuzo Akutsu M.D., Ph.D. (Chairman) (Chairman)

  2. Department of Cardiovascular Surgery, The Heart Institute of Japan, Tokyo Women’s Medical College, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162, Japan

    Hitoshi Koyanagi M.D. (Chairman and Professor) (Chairman and Professor)

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© 1998 Springer-Verlag Tokyo

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Kim, H.J., Nakayama, Y., Kim, J., Matsuda, T., Min, B.G. (1998). Surface Fixation of Lumbrokinase via Photochemical Reaction of Azidophenyl Group. In: Akutsu, T., Koyanagi, H. (eds) Heart Replacement. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65921-1_74

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  • DOI: https://doi.org/10.1007/978-4-431-65921-1_74

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-65923-5

  • Online ISBN: 978-4-431-65921-1

  • eBook Packages: Springer Book Archive

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