Abstract
Poly(β-malic acid), PMLA, is known as a worthwhile degradable and bioresorbable drug carrier, which bears pendent reactive carboxylic acid groups usuable to tailor make macromolecular prodrugs. Fluoresceinamine (Fl), has been covalently bound to poly(malic acid) as a model of a drug molecule to prospect the fate of poly(malic acid)-drug conjugate in contact with living cells, and especially cell uptake. For this, Fl was bound to poly(β-malic acid) through amide bound using DCC as the coupling agent. Three partially benzylated Fl-PMLA conjugates were also synthesized by attachement of benzyl alcohol residues to pendent carboxylic acid groups via ester bonds. LASER-microspectrofluorometry (L-MSF) and fluorescence microscopy were used to evaluate the in vitro uptake of the conjugates by tumor cells K562 (A human erythroleukemia). It is shown that cell uptakes are polymer-dependent, molecular weight-dependent and hydrophobe-dependent.
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© 1996 Springer-Verlag Tokyo
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Abdellaoui, K., Boustta, M., Morjani, H., Manfait, M., Vert, M. (1996). Penetration of Polymeric Drug Carriers into K562 Cells. In: Ogata, N., Kim, S.W., Feijen, J., Okano, T. (eds) Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65883-2_11
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DOI: https://doi.org/10.1007/978-4-431-65883-2_11
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-65885-6
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