Regulation of Germline Stem Cells in the Teleost: Gametogenesis, Sex, and Fecundity

Part of the Diversity and Commonality in Animals book series (DCA)


Stem cells are generally defined as the cells that retain themselves while they keep producing differentiating cells. Presence and regulation of stem cells are, therefore, essential for maintaining homeostasis of the tissue. In the gonad, germline stem cells are responsible for producing a tremendous number of gametes at the appropriate time during a reproductive period. In addition, it was recently revealed in teleost fish Medaka that regulation of germline stem cells is related to some other aspects of reproduction. The intrinsic mechanism that determines whether to produce sperm or eggs begins to act in germline stem cells, and the proliferation of germline stem cells causes sex reversal against the direction of the sex determination gene. Furthermore, germline stem cells seem to be involved in fecundity through regulation of the germ cell number and size of regulation. This chapter provides an overall picture of germline cells and discusses their novel roles, which have been mainly revealed through the analysis of the Medaka.


Sex determination Sex reversal Germ cells Medaka 


  1. Aoki et al (2008) Temporal and special localization of three germline-specific proteins in medaka. Dev Dyn 237:800–807CrossRefPubMedGoogle Scholar
  2. Aoki et al (2009) Expression and syntenic analyses of four nanos genes in medaka. Zool Sci 26:112–118CrossRefPubMedGoogle Scholar
  3. Beer, Draper (2013) Nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary. Dev Biol 374:308–311CrossRefPubMedGoogle Scholar
  4. Bellaiche et al (2014) Spermatogonial stem cell quest; nanos2, marker of a subpopulation of undifferentiated A spermatogonia inn trout testis. Biol Reprod 90:1–14CrossRefGoogle Scholar
  5. Froschauer et al (2013) Oct-EGFP reporter gene expression marks the stem cells in embryonic development and in the adult gonads of transgenic medaka. Mol Reprod 80:48–58CrossRefGoogle Scholar
  6. Kawasaki et al (2012) Production of zebrafish offspring from cultured spermatogonial stem cells. Genes Cells 4:316–325CrossRefGoogle Scholar
  7. Klüver et al (2005) Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specificsubfunctionalization. Dev Genes Evol 215:297–305CrossRefPubMedGoogle Scholar
  8. Köprunner et al (2001) A zebrafish nanos-related gene is essential for the development of primordial germ cells. Genes Dev 15:2877–2885PubMedPubMedCentralGoogle Scholar
  9. Kurokawa et al (2006) Time-lapse analysis reveals different modes of primordiall germ cell migrateon in the medaka Oryzias latipes. Dev Growth Differ 48:209–221CrossRefPubMedGoogle Scholar
  10. Kurokawa et al (2007) Germ cells are essential for sexual dimorphism in the medaka gonad. Proc Natl Acad Sci U S A 104:16958–16963CrossRefPubMedPubMedCentralGoogle Scholar
  11. Lee et al (2013) Nanog, Pou5f1 and Soxb1 activate zygotic gene expression during the maternal-to-zygotic transition. Nature 503:360–364CrossRefPubMedPubMedCentralGoogle Scholar
  12. Lei, Spradling (2013) Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial germ follicles. Proc Natl Acad Sci U S A 110:8585–8590CrossRefPubMedPubMedCentralGoogle Scholar
  13. Morinaga et al (2007) The hotei mutation of medaka in the anti-Müllerian hormone receptor causes the dysregulation of germ cell and sexual development. Proc Natl Acad Sci U S A 104:9691–9696CrossRefPubMedPubMedCentralGoogle Scholar
  14. Nakamura et al (2010) Identification of germline stem cells in the ovary of teleost fish, medaka (Oryzias latipes). Science 328:1561–1563CrossRefPubMedGoogle Scholar
  15. Nakamura et al (2012a) Hyperproliferation of mitotically active germ cells due to defective anti-Müllerian hormone signaling mediates sex reversal in medaka. Development 139:2283–2287CrossRefPubMedGoogle Scholar
  16. Nakamura et al (2012b) Analysis of medaka sox9 orthologue reveals a conserved role in germ cell maintenance. PLoS One 7:e29928CrossRefGoogle Scholar
  17. Neumann et al (2011) Zebrafish models of germ cell tumor. Methods Cell Biol 105:3–24PubMedPubMedCentralGoogle Scholar
  18. Nishimura, Tanaka (2014) Gonadal development in fish. Sex Dev 8:252–261CrossRefPubMedGoogle Scholar
  19. Nishimura et al (2015) foxl3 is a germ cell-intrinsic factor involved in sperm-egg fate decision in medaka. Science 349:328–331CrossRefPubMedGoogle Scholar
  20. Nóbrega et al (2010) Spermatogonial stem cell niche and spermatogonia transplantation in zebrafish. PLoS One 5:e1112808CrossRefGoogle Scholar
  21. Okutsu et al (2006) Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish. Proc Natl Acad Sci U S A 103:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  22. Ozaki et al (2011) Patterns evaluation of Sycp3, Plzf and CyclinB3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. Gene Expr 11:309–311CrossRefGoogle Scholar
  23. Rodriguez-Mari et al (2010) Sex reversal in zebrafish fancl mutants is caused by Tp53-mediated germ cell apoptosis. PLoS Genet 6:e1001034CrossRefPubMedPubMedCentralGoogle Scholar
  24. Sada et al (2012) NANOS2 acts downstream of glial cell line-derived neurotrophic factor signaling to suppress differentiation of spermatogonial stem cells. Stem Cells 30:280–291CrossRefPubMedGoogle Scholar
  25. Saito et al (2008) Proliferation of germ cells during gonadal sex differentiation in medaka: insights from germ cell-depleted mutant zenzai. Dev Biol 310:280–290CrossRefGoogle Scholar
  26. Sánchez-Sánchez et al (2010) Medaka oct4 is expressed in early embryo development and in primordial germ cells and sult gonads. Dev Dyn 239:672–679CrossRefPubMedGoogle Scholar
  27. Tanaka (2009) Chapter 5.2.5. ‘Gonads’. In: Kinoshita, Murata, Naruse and Tanaka (eds.) Medaka – biology, management, and experimental protocols. Wiley-Blackwell, IOWA, USAGoogle Scholar
  28. Tanaka (2013) Vertebrate female germline – acquisition of femaleness. WIREs Dev Biol.
  29. Tanaka et al (2001) Establishment of medaka transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells. Proc Natl Acad Sci U S A 98:2544–2549CrossRefPubMedPubMedCentralGoogle Scholar
  30. Wang et al (2011) Identification of pluripotency genes in the fish medaka. Int J Biol Sci 7:440–451CrossRefPubMedPubMedCentralGoogle Scholar
  31. Wong, Collodi (2013) Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture. PLoS One 8:e71332CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of ScienceNagoya UniversityNagoyaJapan

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