Bioelectrics pp 155-274 | Cite as

Biological Responses

  • Ken-ichi YanoEmail author
  • Lea Rems
  • Tadej Kotnik
  • Damijan Miklavčič
  • James C. Weaver
  • Kyle C. Smith
  • Reuben S. Son
  • Thiruvallur R. Gowrishankar
  • P. Thomas Vernier
  • Zachary A. Levine
  • Marie-Pierre Rols
  • Justin Teissie
  • Lluis M. Mir
  • Andrei G. Pakhomov
  • Peter Nick
  • Wolfgang Frey
  • David A. Dean
  • Keiko Morotomi-Yano
  • Robert E. NealII
  • Suyashree Bhonsle
  • Rafael V. Davalos
  • Stephen J. Beebe


Cells are the structural and functional unit of all living organisms and exhibit fundamental properties of life. Cells are surrounded by the cell membrane and subdivided into various compartments. Pulsed electric fields (PEFs) exert profound effects on cells by interacting with the cell membrane and other cellular components. This chapter describes the biological effects of PEF at cellular and subcellular levels. First, this chapter begins with the overview of cell exposure to PEF from a biophysical point of view. Second, the interaction of PEF with biological membranes, membrane pore formation, and their physiological significance is described from multifaceted standpoints. Next, this chapter explains subcellular events induced by PEF, including the effect on cytoskeleton and signal transduction. Lastly, detailed description on irreversible electroporation and cell death by PEF is provided. The topics covered in this chapter serve as the basis for the applications of PEF in medicine, environmental science, and food and biomass processing.


Electroporation Electropermeabilization Computational model Cell membrane Cellular effect Cell death 


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Copyright information

© Springer Japan 2017

Authors and Affiliations

  • Ken-ichi Yano
    • 1
    Email author
  • Lea Rems
    • 2
  • Tadej Kotnik
    • 2
  • Damijan Miklavčič
    • 2
  • James C. Weaver
    • 3
  • Kyle C. Smith
    • 3
  • Reuben S. Son
    • 3
  • Thiruvallur R. Gowrishankar
    • 3
  • P. Thomas Vernier
    • 4
  • Zachary A. Levine
    • 5
  • Marie-Pierre Rols
    • 6
  • Justin Teissie
    • 6
  • Lluis M. Mir
    • 7
  • Andrei G. Pakhomov
    • 4
  • Peter Nick
    • 8
  • Wolfgang Frey
    • 9
  • David A. Dean
    • 10
  • Keiko Morotomi-Yano
    • 11
  • Robert E. NealII
    • 12
  • Suyashree Bhonsle
    • 13
  • Rafael V. Davalos
    • 13
  • Stephen J. Beebe
    • 4
  1. 1.Kumamoto UniversityKumamotoJapan
  2. 2.Faculty of Electrical EngineeringUniversity of LjubljanaLjubljanaSlovenia
  3. 3.Harvard-MIT Division of Health Sciences and TechnologyMassachusetts Institute of TechnologyCambridgeUSA
  4. 4.Frank Reidy Research Center for BioelectricsOld Dominion UniversityNorfolkUSA
  5. 5.Department of PhysicsUniversity of California Santa BarbaraSanta BarbaraUSA
  6. 6.Institute of Pharmacology and Structural BiologyCNRS and University of ToulouseToulouseFrance
  7. 7.Vectorology and Antitumor TherapiesUMR 8203, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gustave RoussyVillejuifFrance
  8. 8.Botanical InstituteKarlsruhe Institute of TechnologyKarlsruheGermany
  9. 9.Institute for Pulsed Power and Microwave TechnologyKarlsruhe Institute of TechnologyEggenstein-LeopoldshafenGermany
  10. 10.Department of PediatricsUniversity of RochesterRochesterUSA
  11. 11.Institute of Pulsed Power ScienceKumamoto UniversityKumamotoJapan
  12. 12.AngioDynamics Inc.QueensburyUSA
  13. 13.Biomedical EngineeringVirginia TechBlacksburgUSA

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