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Biopharmaceutical Evaluation of Intermolecular Interactions by AUC-SE

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Analytical Ultracentrifugation

Abstract

Analytical ultracentrifugation sedimentation equilibrium (AUC-SE) is a useful technique to investigate the weak reversible intermolecular interactions among proteins in solution. It provides biophysical information such as the average apparent molecular weight, stoichiometry, and association constant of associating proteins. Several studies of intermolecular interaction in biopharmaceuticals by AUC-SE are introduced in this chapter. AUC-SE also provides the second virial coefficient (B 2), which represents the type, i.e., repulsive and attractive, and a magnitude of intermolecular interactions. The B 2 values obtained from the protein solution at low concentrations showed good correlation with aggregation and viscosity of MAb at high concentrations, indicating that B 2 can be an effective indicator of aggregation propensity and viscosity. These findings suggest that AUC-SE provides clues to understand the self-association in biopharmaceuticals and to establish effective manufacturing process, formulation, and administration of biopharmaceuticals.

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Author information

Authors and Affiliations

  1. Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., 1-12-1, Shinomiya, Hiratsuka-shi, Kanagawa, 254-0014, Japan

    Shuntaro Saito

  2. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Susumu Uchiyama

Authors
  1. Shuntaro Saito
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  2. Susumu Uchiyama
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Correspondence to Susumu Uchiyama .

Editor information

Editors and Affiliations

  1. Graduate School of Engineering, Osaka University, Suita, Osaka, Japan

    Susumu Uchiyama

  2. Life Science Research Center, Nihon University, Fujisawa, Kanagawa, Japan

    Fumio Arisaka

  3. Department of Neurology, Harvard Medical School, Boston, Massachusetts, USA

    Walter F. Stafford

  4. BITC, University of New Hampshire, Durham, New Hampshire, USA

    Tom Laue

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Saito, S., Uchiyama, S. (2016). Biopharmaceutical Evaluation of Intermolecular Interactions by AUC-SE. In: Uchiyama, S., Arisaka, F., Stafford, W., Laue, T. (eds) Analytical Ultracentrifugation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55985-6_21

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