Advertisement

Ovarian Tissue Cryopreservation: Ovarian Cortical Tissue Vitrification

  • Yodo Sugishita
  • Shu Hashimoto
  • Takayuki Yamochi
  • Suguru Igarashi
  • Mariko Nakajima
  • Chie Nishijima
  • Seido Takae
  • Yuki Horage
  • Kazuhiro Kawaura
  • Yoshihiko Hosoi
  • Yoshiharu Morimoto
  • Nao SuzukiEmail author
Chapter
  • 607 Downloads

Abstract

Vitrification has become common practice in cryopreservation of embryo, blastocyst, oocytes, and sperm. Freezing of ovarian tissue has been studied since the late 1990s, and in 2004, Donnez et al. reported that live births could be achieved with this method. Reports worldwide have indicated that slow freezing is a common method of cryopreservation. Successive reports have emerged since 2005 describing ovarian tissue cryopreservation by vitrification in animal experiments and using human tissue; however, there have been no reports to date in which vitrification was clinically applied and live births were successfully achieved. We have been conducting studies through pre-clinical tests using Cynomolgus monkey (Macaca fascicularis) since 2006, and as a result, at present in June, 2016, we have achieved ovarian tissue cryopreservation in 229 cases and 2 live births following thawed transplantation. This article outlines the research findings so far regarding ovarian tissue cryopreservation by vitrification; additionally, it describes our ovarian cortical tissue vitrification method.

Keywords

Fertility preservation Slow freezing Vitrification Ovarian tissue cryopreservation Ovarian cortical tissue vitrification Ovarian cortex vitrifycation 

References

  1. 1.
    Hoagland H, Pincus G (1942) Revival of mammalian sperm after immersion in liquid nitrogen. J Gen Physiol 25(3):337–344CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    The Practice Committee of the American Society for Reproductive Medicine (2014) Ovarian tissue cryopreservation: a committee opinion. Fertil Steril 101(5):1237–1243CrossRefGoogle Scholar
  3. 3.
    Cakmak H, Audra Katz RN, Cedars MI, Rosen MP (2013) Effective method for emergency fertility preservation: random-start controlled ovarian stimulation. Fertil Steril 100(6):1673–1680CrossRefPubMedGoogle Scholar
  4. 4.
    Oktay K, Nugent D, Newton H, Salha O, Chatterigee P, Gosden RG (1997) Isolation and characterizeation of primordiall follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 3:481–486CrossRefGoogle Scholar
  5. 5.
    Oktay K, Newton H, Aubanrd Y, Salha O, Gosden RG (1998) Cryopreservation of immature human oocytes and ovarian tissue: an emerging technology? Fertil Steril 59(1):1–7CrossRefGoogle Scholar
  6. 6.
    Newthon H, Aubard Y, Rutherford A, Sharma V, Gosden R (1996) Low temperature storage and grafting of human ovarian tissue. Hum Reprod 11(7):1487–1491CrossRefGoogle Scholar
  7. 7.
    Hovatta O, Silye R, Krausz T, Abir R, Margara R, Trew G, Lass A, Winston RM (1996) Cryopreservation of human ovarian tissue using dimethylsulphoxide and propanediol-sucrose as cryoprotectants. Hum Reprod 11(6):1268–1272CrossRefPubMedGoogle Scholar
  8. 8.
    Donnez J, Bassil S (1998) Indications of cryopreservation of ovarian tissue. Hum Reprod Update 4(3):248–259CrossRefPubMedGoogle Scholar
  9. 9.
    Meirow D, Ben Yehuda D, Prus D, Poliack A, Schenker JG, Rachmilewiz EA, Lewin A (1998) Ovarian tissue banking in patients with Hodgkin’s disease: is it safe? Fertil Steril 69(6):996–998CrossRefPubMedGoogle Scholar
  10. 10.
    Oktay K, Buyuk E, Veeck L, Zaninovic N, Xu K, Takeuchi T, Opsahl M, Rosenwaks Z (2004) Embryo development after heterotopic transplantation of cryopreserved ovarian tissue. Lancet 363:837–840CrossRefPubMedGoogle Scholar
  11. 11.
    Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, Van Langendonckt A (2004) Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet 364:1405–1410CrossRefPubMedGoogle Scholar
  12. 12.
    Callejo J, Salvador C, Miralles A et al (2001) Long-term ovarian function evaluation after autografting by implantation with fresh and frozen–thawed human ovarian tissue. J Clin Endocrinol Metab 8:4489–4494CrossRefGoogle Scholar
  13. 13.
    Fabbri R (2006) Cryopreservation of human oocytes and ovarian tissue. Cell Tissue Bank 7:113–122CrossRefPubMedGoogle Scholar
  14. 14.
    Gook DA, McCully BA, Edgar DH, McBain JC (2001) Development of antral follicles in human cryopreserved ovarian tissue following xenografting. Hum Reprod 16:417–422CrossRefPubMedGoogle Scholar
  15. 15.
    Gook DA, Edgar DH, Borg J et al (2003) Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovar- ian tissue following xenografting. Hum Reprod 18:1772–1781CrossRefPubMedGoogle Scholar
  16. 16.
    Gook DA, Edgar DH, Borg J et al (2005) Diagnostic assess- ment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 26:72–78Google Scholar
  17. 17.
    Kim SS, Hwang IT, Lee HC, Kim DH (2003) Ovarian function after autotransplantation of frozen thawed human ovarian tissue: an experience of human ovarian grafting into two eterotopic sites. Fertil Steril 80:94–95CrossRefGoogle Scholar
  18. 18.
    Kim SS, Hwang IT, Lee HC (2004) Heterotopic autotrans- plantation of cryobanked human ovarian tissue as a strategy to restore ovarian function. Fertil Steril 82:930–932CrossRefPubMedGoogle Scholar
  19. 19.
    Kim SS, Lee WS, Chung MK et al (2009) Long-term ovarian function and fertility after heterotopic autotransplantation of cryobanked human ovarian tissue: 8-year experience in cancer patients. Fertil Steril 91:2349–2354CrossRefPubMedGoogle Scholar
  20. 20.
    Meirow D, Levron J, Eldar-Geva T et al (2005) Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med 353:318–321CrossRefPubMedGoogle Scholar
  21. 21.
    Meirow D, Levron J, Eldar-Geva T et al (2007) Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth. Fertil Steril 87:418.e7–418.e15CrossRefGoogle Scholar
  22. 22.
    Von Wolff M, Donnez J, Hovatta O et al (2009) Cryopreserva- tion and autotransplantation of human ovarian tissue prior to cytotoxic therapy – a technique in its infancy but already successful in fertility preservation. Eur J Cancer 45:1547–1553CrossRefGoogle Scholar
  23. 23.
    Stoop D, Cobo A, Silber S (2014) Fertility preservation for age-related fertileity decline. Lancet 384:1311–1319CrossRefPubMedGoogle Scholar
  24. 24.
    Donnez J, Dolmans MM, Pellicer A, Diaz-Garcia C, Ernst E, Macklon KT, Andersen CY (2015) Fertility preservation for age-related fertility decline. Lancet 385:506–507CrossRefPubMedGoogle Scholar
  25. 25.
    von Wolff M, Montag M, Dittrich R, Denshlag D, Nawroth F, Lawrenz B (2011) Fertility preservation in women-a practiceal guide to preservation techniques and therapeutic strategyes in breast cancer, Hodgkin’s lymphoma and borderline ovaryian tumors by the fertility preservation network FertiPROTEKT. Arch Gynecol Obstet 284:427–435CrossRefGoogle Scholar
  26. 26.
    ISFP Practiee Committee, Kim SS, Donnez J, Barri P, Pellicer A, Pasquale P, Rosenwaks Z, Nagy P, Falcone T, Andersen C, Hovatta O, Wallace H, Meirow D, Gook D, Kim SH, Tzeng C-R, Suzuki S, Ishizuka B, Dolmans M-M (2012) Recommendations for fertileity preservation in patients with lymphoma, leukemia, and breast cancer. J Assist Reprod Genet 29:465–468CrossRefGoogle Scholar
  27. 27.
    Loren AW, Mangu PB, Beck LN, Brennan L, Magdalinski AJ, Partridge AH, Quinn G, Wallace WH, Oktay K (2013) Fertility preservation for patients with cancer: American Society of Clinical Oncology Clinical Practice Guideline Update. J Clin Oncol 311(19):2500–2511CrossRefGoogle Scholar
  28. 28.
    Featured Update to the NCCN Guidelines (2014) Adolescent and Young Adult Oncology, Ver 2.2014. J Natl Compr Cancer Netw. 12(1):21–32Google Scholar
  29. 29.
    Yeoman RR, Wolf DP, Lee DM (2005) Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing. Fertil Steril 83:1248–1254CrossRefPubMedGoogle Scholar
  30. 30.
    Gandolfi F, Paffoni A, Papasso Brambilla E, Bonetti S, Brevini TAL, Pharm D, Ragni G (2006) Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models. Fertil Steril 85(1):1150–1156CrossRefPubMedGoogle Scholar
  31. 31.
    Ting AY, Yeoman RR, Lawson S, Zelinski MB (2011) In vitro development of secondary follicles from cryopreserved rhesus macaque ovarian tissue after slow-rate-freeze or vitrification. Hum Reprod 26(9):2461–2472CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Keros V, Xella S, Hultenby K, Pettersson K, Sheikhi M, Volpe A, Hreinsson J, Hovatta O (2009) Vitrification versus controlled-rate freezing in cryopreserveation of human ovarian tissue. Hum Reprod 24(7):1670–1683CrossRefPubMedGoogle Scholar
  33. 33.
    Sheikhi M, Hultendby K, Niklasson B, Lundqvist M, Hobatta O (2011) Clinical grade vitrification: an ultrastructural analysis of follicles and stroma in vitrified tissue. Hum Reprod 26(3):594–603CrossRefPubMedPubMedCentralGoogle Scholar
  34. 34.
    Amorim CA, Curaba M, Van Langendonckt A, Dolmans M-M, Donnez J (2011) Vitrification as analternative means of cryopreserveing ovarian tissue. Repro Biomed On 23:160–186CrossRefGoogle Scholar
  35. 35.
    Hashimoto S, Suzuki N, Yamanaka M, Hosoi Y, Ishizuka B, Morimoto Y (2010) Effects of vitrification solutions and equilibrateon times on the morphology of cynomolgus ovaryan tissue. Repro Bio Online 21:501–509CrossRefGoogle Scholar
  36. 36.
    Igarashi S, Suzuki N, Hashimoto S, Takae S, Takenoshita M, Hosoi Y, Morimoto Y, Ishizuka B (2010) Heterotopic autotransplantation of ovarian cortex in cynomolgus monkeys. Hum Cell 23(1):26–34PubMedGoogle Scholar
  37. 37.
    Ishimori H, Takahashi Y, Kanagawa H (1992) Factors affecting survival of mouse blastocysts vitrified by a mixture of ethylene glycol and dimethyl sulfoxide. Theriogenology 38:1175–1185CrossRefPubMedGoogle Scholar
  38. 38.
    Suzuki N, Hashimoto S, Igarashi S, Takae S, Yamanaka M, Yamochi T, Takenosita M, Hosoi Y, Morimoto Y, Ishizuka B (2012) Assesment of long-term function of heterotopic transplants of vitrified ovarian tissue in cynomolgus monkeys. Hum Reprod 27(8):2420–2429CrossRefPubMedGoogle Scholar
  39. 39.
    Hashimoto S, Suzuki N, Amo A, Yamochi T, Hosoi Y, Morimoto Y (2013) Good thermally conducting material supports follicle morphologyes of procine ovaries cryopreserved with ultrarapid vitrifycation. J Reprod Dev 59(5):496–499CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Kawamura K, Cheng Y, Suzuki N, Deguchi M, Sato Y, Takae S, Ho CH, Kawamura N, Tamura M, Hashimoto S, Sugishita Y, Morimoto Y, Hosoi Y, Yoshioka N, Ishizuka B, Hsueh AJ (2013) Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci 110(43):17474–17479CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Suzuki N, Yoshioka N, Takae S, Sugishita Y, Tamura M, Hashimoto S, Morimoto Y, Kawamura K (2015) Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency. Hum Reprod 30:608–615CrossRefPubMedGoogle Scholar
  42. 42.
    Revel A, Elami A, Bor A, Yavin S, Natan Y, Arav A (2004) Whole sheep ovary cryopreservation and transplantation. Fertil Steril 82:1714–1715CrossRefPubMedGoogle Scholar
  43. 43.
    Courbiere B, Massardier J, Salle B, Mazoyer C, Guerin JF, Lornage J (2005) Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification. Fertil Steril 84:1065–1071CrossRefPubMedGoogle Scholar
  44. 44.
    Courbiere B, Odagescu V, Baudot A, Massardier J, Mazoyer C, Salle B, Lornage J (2006) Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols. Fertil Steril 86:1243–1251CrossRefPubMedGoogle Scholar
  45. 45.
    Courbiere B, Caquant L, Mazoyer C, Franck M, Lornage J, Salle B (2009) Difficulties improving ovarian functional recovery by microvascular transplantation and whole ovary vitrification. Fertil Steril 91:2697–2706CrossRefPubMedGoogle Scholar
  46. 46.
    Silber S, Kagawa N, Kuwayama M, Gosden R (2010) Duration of fertileity after fresh and frozen ovary transplantation. Fertil Steril 94:2191–2196CrossRefPubMedGoogle Scholar
  47. 47.
    Kikuchi I, Kagawa N, Silber S, Kuwayama M, Takehara Y, Aono F, Kumakiri J, Kato O, Takeda S (2013) Oophorectomy for fertility preservation via reduced-port laparoscopic surgery. Surg Innov 20(3):219–224CrossRefPubMedGoogle Scholar
  48. 48.
    Kagawa N, Kikuchi I, Kuwayama M (2015) Ovarian tissue vitrification for fertility preservation. Vitrification in Assisted Reproduction-Springer. 43–49Google Scholar
  49. 49.
    Bielanski A (2014) Biosafety embryo and semen cryopreservation, storage, management and transport. Adv Exp Med Biol 753:429–465CrossRefPubMedGoogle Scholar

Copyright information

© Springer Japan 2016

Authors and Affiliations

  • Yodo Sugishita
    • 1
  • Shu Hashimoto
    • 2
  • Takayuki Yamochi
    • 2
  • Suguru Igarashi
    • 1
  • Mariko Nakajima
    • 1
  • Chie Nishijima
    • 1
  • Seido Takae
    • 1
  • Yuki Horage
    • 1
  • Kazuhiro Kawaura
    • 1
  • Yoshihiko Hosoi
    • 3
  • Yoshiharu Morimoto
    • 2
    • 4
  • Nao Suzuki
    • 1
    Email author
  1. 1.Department of Obstetrics and GynecologySt. Marianna University School of MedicineKawasakiJapan
  2. 2.IVF Namba ClinicOsakaJapan
  3. 3.Institute of Advanced TechnologyKinki UniversityWakayamaJapan
  4. 4.Horac Grand Front Osaka ClinicOsakaJapan

Personalised recommendations