Ovarian Tissue Cryopreservation: Slow Freezing

  • Sonia Herraiz
  • Cesar Diaz-GarciaEmail author
  • Antonio Pellicer


The needs for fertility preservation (FP) have substantially increased due to improvements in early diagnosis and survival of many malignancies experienced during the last decades. Different FP options can be offered nowadays, but ovarian tissue cryopreservation and retransplantation is the preferred option in prepubertal girls or when there is an urgent need to start the treatment (no time for ovarian stimulation). In this chapter, the basic principles of cryopreservation will be reviewed. A comprehensive and clinical practice-oriented description of the retrieval, preparation, and slow-freezing procedure of the tissue will be provided, together with a summary of the cryopreservation protocols that have been proven to be effective by obtaining live births.


Cancer Cryopreservation Fertility Ovary 


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Copyright information

© Springer Japan 2016

Authors and Affiliations

  • Sonia Herraiz
    • 1
    • 2
    • 3
  • Cesar Diaz-Garcia
    • 1
    • 3
    Email author
  • Antonio Pellicer
    • 1
    • 2
    • 3
  1. 1.Reproductive Medicine Research GroupIIS La Fe. La Fe University HospitalValenciaSpain
  2. 2.IVI FoundationValenciaSpain
  3. 3.Department of Pediatrics, Obstetrics and GynecologyUniversity of ValenciaValenciaSpain

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