Abstract
In this chapter, we present application of “in vivo cryotechnique” (IVCT) to the injection of glutathione (GSH)-coated quantum dots (QDs), which emit a red-fluorescent signal with an ultraviolet excitation, for kidneys. The frozen kidneys were processed to freeze-substitution fixation (FS) and observed in fluorescence or confocal laser scanning microscope (CLSM). In the renal cortex, QD distribution was detected mostly in glomerular blood capillaries for a few seconds and extended to peritubular blood capillaries at 5 s. By injection of horseradish peroxidase (HRP) and QDs at the same time, the distribution of HRP in the renal cortex at 30 s was detected in the interstitium in addition to blood vessels, whereas QDs were localized only inside blood vessels. Thus, strict time-dependent visualization of blood flow in tissue sections became possible within seconds by combination of IVCT and injection of QDs.
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References
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Terada, N., Saitoh, Y., Ohno, N., Ohno, S. (2016). Application of “In Vivo Cryotechnique” to Visualization of Microvascular Blood Flow in Mouse Kidney by Quantum Dot Injection. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_42
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DOI: https://doi.org/10.1007/978-4-431-55723-4_42
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-55722-7
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