Diverse technological purposes of bread wheat grain require a broadening the genetic base of selection for quality traits. The wild cereal species are widely used for improvement of bread wheat in respect to resistance to fungus diseases through a wide hybridization. The resulted genotypes often carry the alien genetic material with known chromosomal location and rearrangements indicated with molecular markers. Such introgressions potentially may affect technological properties of grain and flour and add to a genetic variability of the trait. Alien cereal species often have a high protein and gluten in grain but, unfortunately, the quality of their gluten is low. Often it has dark unattractive color and sticky in a consistence. For these negative features introgression lines are not considered as possible sources of genetic variability for quality traits. At the same time, the limited introgressions may positively effect on technological properties of grain and flour. It was demonstrated with discovery and investigation of Gpc-B1 gene for high protein content in grain introgressed from Triticum dicoccoides into durum and bread wheat (Carter et al. 2012). The aim of this work was to investigate the influence of introgressions from exotic tetraploid wheat Triticum timopheevii and wild cereal species, Aegilops speltoides and Aegilops markgrafii into bread wheat on grain quality.

The collection of lines with introgressions from T. timopheevii were obtained by crossing and one backcrossing with several spring bread wheat cultivars followed by multiply selection on resistance to fungi diseases (Budashkina 1988). Two lines, 821 and 842, were obtained on the genetic background of cv. Saratovskaya 29 (S29). As was determined using microsatellite markers they carry very similar fragments of introgressions (Leonova et al. 2001). The line 821 carries an entire short arm introgression in 2A chromosome while the line 842 carries a shorter fragment which does not include the distal markers Xgwm636 and Xgwm296. Gluten content in grain was studied in the lines and their parents using a standard method of washing in tap water (Anonymous 1988). Compare of the lines with the parents showed that donor had the highest gluten content, about 57 %, while the recipient cv. S29 had a significantly lower meaning, 34.5 % (Fig. 32.1a). From the two lines, the line 821 had high gluten content (45 %) while the line 842 had the gluten content comparable with the recipient S29. It was supposed that the gene responsible for high gluten content in grain introgressed from T. timopheevii is situated in a distal part of 2AS chromosome in the region of microsatellite marker Xgwm636.

Fig. 32.1
figure 1

Wet gluten content (GC) in grain of bread wheat lines with introgressions from wild cereals: (a) introgression lines 842 (low GC) and 821 (high GC), cv. S29 (recipient), T. timopheevii (donor); (b) cv. S29 (recipient), introgression line 821 (high GC), substitution line S29 (821 2A); (c) introgression line M483/98, cv. Alcedo (recipient), Ae. markgrafii (donor)

To verify this, the recombinant 2A chromosome was introduced from the line 821 again in the genetic background of S29. The line was crossed and backcrossed to the monosomic line for 2A chromosome of the recipient and the disomic population was developed. Again gluten content in grain was studied in the new line. It was found (Fig. 32.1b) that the gluten content was significantly higher in the line than in the recipient and was comparable to 821 line.

Introgression with the similar position on 2AS chromosome was detected in the line M483/98 obtained by crossing of winter cultivar Alcedo and Aegilops markgrafii (Weidner 2004). The introgression includes the region of Xgwm636 and limited by Xgwm296 marker (Iqba et al. 2007). Again the wild cereal had the highest gluten content in grain, more than 40 % (Fig. 32.1c). The mean of the trait in the line 483/98 did not reach the donor meaning but it was on 10 % higher than in the parental cultivar Alcedo. The difference was statistically significant. Now this chromosome is being introduced from introgression line into S29 background in order to compare its effect with introgression from T. timopheevii.

Earlier, the quantitative trait locus QPro.inra-2A for a high protein content in grain was positioned in the region of Xgwm636 using the mapping population of bread wheat Renan × Recital (Groos et al. 2003). The donor of high meaning of the trait was cv. Renan. A close correlation of protein content in grain with gluten content and the similarity of the marker positions associated with manifestation of the traits in different genetic material suggest the presence of the responsible locus on the short arm of 2A chromosome.

Milling parameters are determined by endosperm texture of wheat grain. Vitreous grains give a hard-grain flour with big particles and floury grain gives soft flour with small particles. Different kinds of flour are used for different technological purposes (Peña 2002). Today all the variability is provided by mutations in one locus Ha on 5DS chromosome (Morris 2002). Milling parameters show a quantitative variability but usually cultivars retain the certain characteristics of the trait.

The effect of introgression of 5S chromosome from Ae. speltoides on expression of milling parameters was studied in the work. Vitreousness was determined on cut halves of grains visually; flour particle size was determines instrumentally using PSH-4 device (Anonymous 1988). The line 84/98w from ‘Arsenal’ collection (Lapochkina et al. 2003) obtained from a wide cross of Ae. speltoides with spring wheat cultivar ‘Rodina’ was used. Winter growth habit and presence of awns indicated the introgression into 5A chromosome. Additionally, the line was characterized with a significantly lower vitreousness of endosperm and smaller flour particle size comparing to the initial hard-grained cultivar (Table 32.1).

Table 32.1 The effect of introgression from Ae. Speltoides on milling parameters of wheat flour

In order to prove that just this introgression is responsible for changes in milling parameters this chromosome was introduced into two hard-grain cultivars S29 and Diamant 2 using monosomic lines for 5A chromosomes. It was found that this chromosome significantly decreases vitreousness and particle size to the meanings compared with the meaning of the introgression line (Table 32.2). The introgressed gene was designated with a temporarily gene symbol Ha-Sp (Pshenichnikova et al. 2010).

Table 32.2 Effect of introducing of 5S chromosome of Ae. speltoides into genetic backgrounds of hard-grain cultivars S29 and Diamant 2 on milling parameters of grain

Crossing of introgression line with cultivar Chinese Spring – carrier of the dominant allele of Ha gene in 5DS chromosome resulted in emergence of wide variability in milling parameters relatively parental forms (Fig. 32.2). Among them the plants were found carrying two dominant genes for grain softness with new milling properties – very low vitreousness (0–30 %) and small particle size (11–12 μm). These plants have become the progenitors of F6-F7 lines which retain these properties. Their flour may be used for special technological purposes.

Fig. 32.2
figure 2

Milling parameters of F6–7 lines carrying two dominant genes Ha and Ha-Sp for soft endosperm texture