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Cell Scooper: A Device for the Rapid Transfer of Living Cell Sheet

  • Kenjiro Tadakuma
  • Nobuyuki Tanaka
  • Yuji Haraguchi
  • Mitsuru Higashimori
  • Makoto Kaneko
  • Tatsuya Shimizu
  • Masayuki YamatoEmail author
  • Teruo Okano
Chapter

Abstract

In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7 ± 1.6 s) using the device compared with that of a conventional method using a pipette (430 ± 180 s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cell-cell junctions and ion channel proteins on the cell opsurface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine.

Keywords

Mechanism Device Scooping Transfer 

Notes

Acknowledgement

Upon the development of the cell-sheet transfer/transplantation device in this study, the authors referred the basic concept of SWTL, a food-handling devise, developed by Furukawakikou Co., LTd. (Niigata, Japan). The authors are grateful to the members of Furukawakikou. This work was supported by grants from a new area of Hyper Bio Assembler for 3D Cellular Systems (BioAssembler) Project, a Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) Fellows (23•7758), the Global Center of Excellence Program, Multidisciplinary Education and Technology and Research Center for Regenerative Medicine (MERCREM), Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science, and the High-Tech Research Center Program from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, and JSPS through the “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program),” initiated by the Council for Science and Technology Policy (CSTP).

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Copyright information

© Springer Japan 2015

Authors and Affiliations

  • Kenjiro Tadakuma
    • 2
  • Nobuyuki Tanaka
    • 1
  • Yuji Haraguchi
    • 1
  • Mitsuru Higashimori
    • 2
  • Makoto Kaneko
    • 2
  • Tatsuya Shimizu
    • 1
  • Masayuki Yamato
    • 1
    Email author
  • Teruo Okano
    • 1
  1. 1.Graduate School of MedicineTokyo Women’s Medical University TokyoTokyoJapan
  2. 2.Graduate School of EngineeringOsaka UniversityTokyoJapan

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