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Genome Editing in Zebrafish and Medaka

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Book cover Targeted Genome Editing Using Site-Specific Nucleases

Abstract

Two small ornamental fish, zebrafish and medaka, have become popular model vertebrates suitable for genetic analysis. A number of mutant fish defective in organogenesis and biological responses have been isolated from chemical mutagenesis screens and forward genetic analyses of these mutants have identified novel essential regulators involved in morphological and physiological processes. Recently, remarkable innovations in genome editing technologies, such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) as well as the clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system, have enabled us to introduce double-strand DNA breaks at target genomic loci, subsequently leading to the disruption of targeted genes (knockout) or their replacement with homologous fragments (knockin). Here, we summarize the usefulness and application of targeted genome modifications in zebrafish and medaka and their relevance to the basic and medical sciences.

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Abbreviations

CRISPR:

clustered interspaced short palindromic repeats

TALEN:

transcription activator-like effector nuclease

ZFN:

zinc finger nuclease

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Acknowledgements

We would like to thank Drs. Ota S. and Hisano Y. for valuable discussion. This work was supported by the Funding Program for Next Generation World-Leading Researchers (NEXT Program) and by the Japan Society for the Promotion of Science (JSPS), and a Grant-in-Aid for JSPS Fellows (S.A.).

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Authors and Affiliations

  1. Laboratory for Developmental Biology, Center for Medical Education and Sciences, Graduate School of Medical Science, University of Yamanashi, Simogatou 1111, Chuo, Yamanashi, 409-3898, Japan

    Atsuo Kawahara

  2. Okazaki Institute for Integrative Bioscience, National Institute of Natural Sciences, Okazaki, Aichi, 444-8787, Japan

    Taijiro Yabe & Shinji Takada

  3. Department of Basic Biology, The Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, 444-8787, Japan

    Taijiro Yabe & Shinji Takada

  4. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo, Kyoto, Kyoto, 626-8502, Japan

    Satoshi Ansai & Masato Kinoshita

Authors
  1. Atsuo Kawahara
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  2. Taijiro Yabe
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  3. Satoshi Ansai
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  4. Shinji Takada
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  5. Masato Kinoshita
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Corresponding author

Correspondence to Atsuo Kawahara .

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Editors and Affiliations

  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

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Kawahara, A., Yabe, T., Ansai, S., Takada, S., Kinoshita, M. (2015). Genome Editing in Zebrafish and Medaka. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_8

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