Hepatic Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by Two- and Three-Dimensional Culture Systems In Vitro

Part of the Nanomedicine and Nanotoxicology book series (NANOMED)


Hepatocytes differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) have a wide range of potential applications in biomedical research, drug discovery, and the treatment of liver disease. In this review, we provide an up-to-date overview of the wide variety of hepatic differentiation protocols. Moreover, we discuss the application of these protocols to three-dimensional culture systems in an attempt to induce hepatocyte-like cells with high hepatic functions.


Differentiation ES cells Hepatocytes iPS cells Three-dimensional culture 


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Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Laboratory of Biochemistry and Molecular BiologyGraduate School of Pharmaceutical Sciences, Osaka UniversityOsakaJapan
  2. 2.Laboratory of Hepatocyte DifferentiationNational Institute of Biomedical InnovationOsakaJapan
  3. 3.The Center for Advanced Medical Engineering and InformaticsOsaka UniversityOsakaJapan
  4. 4.iPS Cell-Based Research Project on Hepatic Toxicity and MetabolismGraduate School of Pharmaceutical Sciences, Osaka UniversityOsakaJapan

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