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Hepatic Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by Two- and Three-Dimensional Culture Systems In Vitro

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Engineered Cell Manipulation for Biomedical Application

Part of the book series: Nanomedicine and Nanotoxicology ((NANOMED))

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Abstract

Hepatocytes differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) have a wide range of potential applications in biomedical research, drug discovery, and the treatment of liver disease. In this review, we provide an up-to-date overview of the wide variety of hepatic differentiation protocols. Moreover, we discuss the application of these protocols to three-dimensional culture systems in an attempt to induce hepatocyte-like cells with high hepatic functions.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, 565-0871, Japan

    Maiko Higuchi & Hiroyuki Mizuguchi

  2. Laboratory of Hepatocyte Differentiation, National Institute of Biomedical Innovation, Osaka, 567-0085, Japan

    Hiroyuki Mizuguchi

  3. The Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, 565-0871, Japan

    Hiroyuki Mizuguchi

  4. iPS Cell-Based Research Project on Hepatic Toxicity and Metabolism, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, 565-0871, Japan

    Hiroyuki Mizuguchi

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  1. Maiko Higuchi
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Correspondence to Hiroyuki Mizuguchi .

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Editors and Affiliations

  1. Osaka University, Osaka, Japan

    Misturu Akashi

  2. Osaka University, Osaka, Japan

    Takami Akagi

  3. Osaka University, Osaka, Japan

    Michiya Matsusaki

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Higuchi, M., Mizuguchi, H. (2014). Hepatic Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by Two- and Three-Dimensional Culture Systems In Vitro. In: Akashi, M., Akagi, T., Matsusaki, M. (eds) Engineered Cell Manipulation for Biomedical Application. Nanomedicine and Nanotoxicology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55139-3_7

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