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Engineering Cellular Assembly for Applications in Regenerative Medicine

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Engineered Cell Manipulation for Biomedical Application

Part of the book series: Nanomedicine and Nanotoxicology ((NANOMED))

Abstract

In vitro cell culture methods are undergoing a revolutionary transition towards 3D systems that better imitate the complex microenvironment a cell experiences in the body. Cells must be permitted to participate in an intricate communication network via biochemical and mechanical signaling with neighboring cells and key components of the extracellular matrix, something that is inherently lacking in monolayer cultures using petri dishes or flasks. Over the last 2–3 decades, a number of 3D cell culture techniques have been introduced that rely on cell self-organizing capacity such as hanging drops, centrifuged pellets, and suspension culture. However, more recent approaches have emerged to further control cellular organization in 3D. Methods for engineering a directed assembly of cells will yield 3D models that more closely resemble their organ mimics, which would have significant implications in the field of regenerative medicine.

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Acknowledgments

Work was funded by the Swiss National Science Foundation (CR32I3-146338/1), the European Union Seventh Framework Programme (FP7/2007–2013) under grant agreement n0 NMP4-SL-2009-229292, and an AO Foundation startup grant (S-11-60Z).

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Authors and Affiliations

  1. Cartilage Engineering+Regeneration, Swiss Federal Institute of Technology, ETH Zürich, Otto-Stern-Weg 7, 8093, Zürich, Switzerland

    Christopher Millan & Marcy Zenobi-Wong

Authors
  1. Christopher Millan
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  2. Marcy Zenobi-Wong
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Correspondence to Marcy Zenobi-Wong .

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Editors and Affiliations

  1. Osaka University, Osaka, Japan

    Misturu Akashi

  2. Osaka University, Osaka, Japan

    Takami Akagi

  3. Osaka University, Osaka, Japan

    Michiya Matsusaki

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© 2014 Springer Japan

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Millan, C., Zenobi-Wong, M. (2014). Engineering Cellular Assembly for Applications in Regenerative Medicine. In: Akashi, M., Akagi, T., Matsusaki, M. (eds) Engineered Cell Manipulation for Biomedical Application. Nanomedicine and Nanotoxicology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55139-3_6

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