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Online Monitoring of Biodegradation Processes Using Enzymatic Biosensors

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Biodegradative Bacteria

Abstract

Biodegradation of organic compounds by natural attenuation or bioaugmentation is widely used for removal of unwanted chemicals from the environment. The essential component of remediation technologies is monitoring of a contaminant levels. Although the analytical methods of gas and liquid chromatography are available, they are time-, labour- and resource-demanding, placing limitations on the number of samples that can be analysed at a time. Furthermore, these methods cannot be easily adapted for in situ measurements. Biosensors can be used as an alternative or complement to these conventional techniques. Biosensors are based on a biological component coupled to a transducer, which translates the interaction between an analyte and a biocomponent into a signal that can be processed. Application of biosensors in monitoring of environmental contaminants is promising owing to their sensitivity, low costs, user-friendliness and adaptability for in situ measurements. In this contribution, we describe development of haloalkane dehalogenase-based biosensors and their application for detection of halogenated hydrocarbons in the environmental samples.

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Abbreviations

CB:

Chlorobutane

CCMP:

3-Chloro-2-(chloromethyl)-1-propene

DBA:

1,2-Dibromoethane

DbjA:

Haloalkane dehalogenase from Bradyrhizobium japonicum USDA110

DCA:

1,2-Dichloroethane

DhaA:

Haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064

DhlA:

Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10

EC:

Enzyme commission number

LED:

Light-emitting diode

LinB:

Haloalkane dehalogenase from Sphingobium japonicum UT26

MW:

Molecular weight

pI:

Isoelectric point

SD:

Standard deviation

TCP:

1,2,3-Trichloropropane

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Acknowledgements

The authors would like to express their sincere thanks to Prof. Ken Reardon (Colorado State University, USA) for introducing us to the field of optical biosensors, Prof. Thomas Scheper (University Hannover, Germany) and Prof. Jaromir Hubalek (Brno University of Technology, Czech Republic) for construction of the first prototypes of our optical biosensors and to Dr. Martin Trtilek (Photon System Instruments, Czech Republic) for their miniaturization. The work was supported by the Grant Agency of the Czech Republic (P207/12/0775, P503/12/0572), the Grant Agency of the Czech Academy of Sciences (IAA401630901) and the European Regional Development Fund (CZ.1.05/2.1.00/01.0001).

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Authors and Affiliations

  1. Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Masaryk University, Kamenice 5/A13, 62500, Brno, Czech Republic

    Sarka Bidmanova, Tana Koudelakova, Jiri Damborsky & Zbynek Prokop

  2. Enantis, Ltd., Palackeho trida 1802/129, 61200, Brno, Czech Republic

    Eva Hrdlickova, Jiri Damborsky & Zbynek Prokop

Authors
  1. Sarka Bidmanova
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  2. Eva Hrdlickova
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  3. Tana Koudelakova
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  4. Jiri Damborsky
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  5. Zbynek Prokop
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Corresponding author

Correspondence to Zbynek Prokop .

Editor information

Editors and Affiliations

  1. Biotechnology Research Center, The University of Tokyo, Tokyo, Japan

    Hideaki Nojiri

  2. Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan

    Masataka Tsuda

  3. Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan

    Masao Fukuda

  4. Bioproduction Research Institute, Nat'l Inst of Adv Industr Sci and Tech, Sapporo, Hokkaido, Japan

    Yoichi Kamagata

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© 2014 Springer Japan

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Bidmanova, S., Hrdlickova, E., Koudelakova, T., Damborsky, J., Prokop, Z. (2014). Online Monitoring of Biodegradation Processes Using Enzymatic Biosensors. In: Nojiri, H., Tsuda, M., Fukuda, M., Kamagata, Y. (eds) Biodegradative Bacteria. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54520-0_8

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