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Imaging Mass Spectrometry pp 11–30Cite as

Guide to Planning the Sample Preparation Step

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Abstract

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS; also referred to as mass spectrometry imaging, MSI) enables the visualization of the distribution of variable biomolecules that have large and varied structures in tissue sections. However, because of such general versatility, the optimization of sample preparation procedure according to each analyte with distinct chemical and physical properties is an important task for IMS, similar to that in traditional MALDI-MS analyses. In this chapter, we briefly introduce the sample preparation strategies of current IMS. We describe the two major strategies for imaging of proteins/peptides and small molecules, such as lipids and drugs. We shall also introduce the representative application example.

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Author information

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan

    Yuki Sugiura

  2. Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Mitsutoshi Setou

Authors
  1. Yuki Sugiura
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  2. Mitsutoshi Setou
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Correspondence to Mitsutoshi Setou .

Editor information

Editors and Affiliations

  1. Molecular Imaging Frontier Research Center Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Mitsutoshi Setou (Professor and Chairman) (Professor and Chairman)

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Sugiura, Y., Setou, M. (2010). Guide to Planning the Sample Preparation Step. In: Setou, M. (eds) Imaging Mass Spectrometry. Springer, Tokyo. https://doi.org/10.1007/978-4-431-09425-8_2

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