Skip to main content

Die Geschichte der Zellkultur

  • Chapter
Book cover Der Experimentator: Zellkultur

Part of the book series: Experimentator ((EXPERIMENTATOR))

  • 21k Accesses

Zusammenfassung

Die ersten Versuche, Gewebe bzw. Organe aus Spenderorganismen in vitrozu kultivieren, liegen schon sehr lange zuruck. Die Anfange der angewandten Gewebe- und Organkultur lassen sich etwa auf den Beginn des zwanzigsten Jahrhunderts zuruckdatieren. Mit der heute schon fast hochtechnisierten Art Zellen zu kultivieren, haben diese ersten Gehversuche nichts gemein. Damals beschaft igten sich nur einige wenige Wissenschaft ler mit solchen Dingen und dies machte sie zu Exoten unter ihren Kollegen. Diese Pioniere kampft en unerbittlich gegen die zahlreichen Kontaminationen, die nicht beherrschbar waren, an. Damals gab es weder Sicherheitswerkbanke, wie wir sie heute kennen, noch hatte man Antibiotika und Antimykotika zur Verfugung, die man zur Vermeidung von Infektionen mit Bakterien und Pilzen ins Nahrmedium geben konnte. Erst mit der Entdeckung des Penicillins Ende der 1920er-Jahre und der Entwicklung wirksamer Antimykotika war es moglich geworden, die Zellkulturplagen in den Griff zu bekommen. Auch die Standardmedien, wie sie heute in jedem Zellkulturlabor benutzt werden, wurden erst spater entwickelt.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.95
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Literatur

  • Allmeling et al. (2007) Wunderwerkstoffder Natur – Spinnenseide in der plastischen Chirurgie. Chem. Unserer Zeit, 2007, 41, 428–434

    Article  CAS  Google Scholar 

  • Blackburn EH, Gall JG (1978) A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol 120: 33–53

    Article  PubMed  CAS  Google Scholar 

  • Bodnar AG et al. (1998) Extension of life span by introduction of telomerase into normal human cells. Science 279: 349–352

    Article  PubMed  CAS  Google Scholar 

  • Carrel A, Ebeling AH (1921) Age and multiplication of fibroblasts. J Exp Med 34: 599–606

    Article  PubMed  CAS  Google Scholar 

  • Carrel A (1912) On the permanent life of tissue outside the organism. J Exp Med 15: 516–528

    Article  PubMed  CAS  Google Scholar 

  • DeLange T et al. (1990) Structure and variability of human chromosome ends. Mol Cell Biol: 10: 518–527

    CAS  Google Scholar 

  • Dimri GP et al. (1995) A biomarker that identifies senescent human cells in culture and in ageing skin in vivo. Proc Natl Acad Sci 92: 9363–9367

    Article  PubMed  CAS  Google Scholar 

  • Evans VJ et al. (1952) A quantitative study of the effect of certain chemically defined media on the proliferation in vitroof strain L cells from the mouse. J Natl Cancer Inst 13: 773–783

    Google Scholar 

  • Evans VJ et al. (1956) Studies of nutrient media for tissue cells in vitro. I. A proteinfree chemically defined medium for cultivation of strain L cells. Cancer Res 16: 77–86

    PubMed  CAS  Google Scholar 

  • Evans VJ et al. (1956) Studies of nutrient media for tissue cells in vitro. II An improved, proteinfree chemically defined medium for longterm culture of strain L929 cells. Cancer Res 16: 87–94

    PubMed  CAS  Google Scholar 

  • Feng F et al. (1995) The RNA component of human telomerase. Science 269: 1236–1241

    Article  PubMed  CAS  Google Scholar 

  • Greider CW, Blackburn EH (1985) Identification of a specific telomere terminal transferase enzyme with two kinds of primer specificity. Cell 51: 405–413

    Article  Google Scholar 

  • Ham RG (1965) Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci 53: 288–293

    Article  PubMed  CAS  Google Scholar 

  • Hanks JH (1957) The future of tissue culture in cancer research. J Natl Cancer Inst 19 (4): 827–832

    PubMed  CAS  Google Scholar 

  • Hanks JH (1957) The future of tissue culture in cancer research: Discussion. J Natl Cancer Inst 19 (4): 833–843

    Google Scholar 

  • Harley CB et al. (1990) Telomeres shorten during ageing of human fibroblasts. Nature 345: 458–460

    Article  PubMed  CAS  Google Scholar 

  • Hastie ND et al. (1990) Telomere reduction in human colorectal carcinoma and with ageing. Nature 346: 866–868

    Article  PubMed  CAS  Google Scholar 

  • Hayflick L, Moorhead P (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25: 585–621

    Article  PubMed  CAS  Google Scholar 

  • Hayflick L (1999) A brief overview of the discovery of cell mortality and immortality and of its influence on concepts about ageing and cancer. Pathol Biol 47: 1094–1104

    PubMed  CAS  Google Scholar 

  • Hayflick L (1998) How and why we age. Exp Gerontol 33: 639–653

    Article  PubMed  CAS  Google Scholar 

  • Hayflick L (1984) The coming of age of WI38. Adv Cell Cult 3: 303–316

    Google Scholar 

  • Hayflick L (1973) The biology of human aging. Am J Med Sci 265: 433–445

    Article  Google Scholar 

  • Hayflick L (1965) The limited in vitrolifetime of human diploid cell strains. Exp Cell Res 37: 614–636

    Article  PubMed  CAS  Google Scholar 

  • Hayflick L et al. (1963) Choice of a cell system for vaccine production. Science 140: 760–763

    Google Scholar 

  • Hayflick L et al. (1962) Preparation of poliovirus vaccines in a human fetal diploid cell strain. Am J Hyg 75: 240–258

    PubMed  CAS  Google Scholar 

  • Herbert BS et al. (1999) Inhibition of telomerase leads to eroded telomeres, reduced proliferation, and apoptosis. Proc Natl Acad Sci 96: 14276–14281

    Article  PubMed  CAS  Google Scholar 

  • Howard A, Pelc SR (1951) Synthesis of nucleoprotein in bean root cells. Nature 167(4250): 599–600

    Article  PubMed  CAS  Google Scholar 

  • Kim NW et al. (1994) Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011–2015

    Article  PubMed  CAS  Google Scholar 

  • Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256(5517): 495–497

    Article  PubMed  CAS  Google Scholar 

  • Lindsey J et al. (1991) In vivoloss of telomere repeats with age in humans. Mutat Res 256: 45–48

    Article  PubMed  CAS  Google Scholar 

  • Littlefield JW (1964) Selection for hybrids from matings of fibroblasts in vitroand their presumed recombinants. Science 145: 709–710

    Article  PubMed  CAS  Google Scholar 

  • McClintock B (1941) The stability of broken ends of chromosomes in Zea mays. Genetics 26: 234–282

    PubMed  CAS  Google Scholar 

  • Morin GB (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59: 521–529

    Article  PubMed  CAS  Google Scholar 

  • Moyzis RK et al. (1988) A highly conserved repetitive DNA sequence (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci 85: 6622–6626

    Article  PubMed  CAS  Google Scholar 

  • Olovnikov AM (1996) Telomeres, telomerase and aging: Origin of the theory. Exp Gerontol 31: 443–448

    Article  PubMed  CAS  Google Scholar 

  • Rubin H (1998) Telomerase and cellular lifespan: ending the debate? Nature Biotechnol 16: 396–397

    Article  CAS  Google Scholar 

  • Shay JW, Gazdar AF (1997) Telomerase in the early detection of cancer. J Clin Path 50: 106–109

    Article  PubMed  CAS  Google Scholar 

  • Shay JW, Wright WE (2000) Hayflick, his limit, and cellular ageing. Nature Mol Cell Biol 1: 72–76

    Article  CAS  Google Scholar 

  • Shay JW, Wright WE (2000) The use of telomerized cells for tissue engineering. Nature Biotechnol 18: 22–23

    Article  CAS  Google Scholar 

  • Witkowski JA (1980) Dr. Carrel's immortal cells. Med Hist 24: 129–142

    Article  PubMed  CAS  Google Scholar 

  • Witkowski JA (1985) The myth of cell immortality. Trends Biochem Sci 10: 258–260

    Article  Google Scholar 

  • Wright WE, Hayflick L (1975) Nuclear control of cellular ageing demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts. Exp Cell Res 96: 113–121

    Article  PubMed  CAS  Google Scholar 

  • Wright WE, Shay JW (2000) Telomere dynamics in cancer progression and prevention: Fundamental differences in human and mouse telomere biology. Nature Med 6: 849–851

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Spektrum Akademischer Verlag Heidelberg

About this chapter

Cite this chapter

Schmitz, S. (2011). Die Geschichte der Zellkultur. In: Der Experimentator: Zellkultur. Experimentator. Spektrum Akademischer Verlag, Heidelberg. https://doi.org/10.1007/978-3-8274-2573-7_1

Download citation

Publish with us

Policies and ethics