Summary
Unique insights into the topography of local metabolism/blood flow interrelationships in focal cerebral ischemia have been afforded by the recent development of powerful image-processing techniques permitting three-dimensional (3D) autoradiographic image-averaging and analysis of replicate studies by a novel method termed “disparity analysis”. This method, based upon a linear affine transformation model, directly estimates scaling, translation and rotation parameters simultaneously. The method was validated in awake Wister rats studied for local cerebral glucose metabolism (1CMRgl) with 14C-2-deoxyglucose. Brains were subserially sectioned, aligned by disparity analysis, and mapped into a common template so as to generate aggregate 3D data sets of the mean and standard deviation of the entire series (n=9). Internal anatomic architecture was faithfully represented in the average image, and Fourier analysis revealed satisfactory retention of low-frequency information. The method was then applied to study metabolism/blood flow relationships in the acute focal ischemic penumbra of Sprague-Dawley rats subjected to distal photothrombotic middle cerebral artery (MCA) occlusion, coupled with permanent ipsilateral and 1 h contralateral common carotid artery occlusions. Matched series were studied for 1CBF at 1.5 h and for 1CMRgl at 1.25–2 h post-occlusion. The averaged 1CBF image revealed the ischemic penumbra (defined as 1CBF 20–40 % of control) to form a “shell” around the cortical ischemic core and a confluent aggregate at the anterior and posterior poles of the corezone. 1CMRgl in the penumbra was heterogeneous, ranging from near-normal to markedly increased. An average 1CMRgl/1CBF ratio data set revealed marked metabolism-flow uncoupling in penumbral pixels, averaging nearly five-fold above control ratio values. Sustained deflections of the DC potential were recorded in the penumbra, the site of marked uncoupling. This analysis defined for the first time the 3D topography of the ischemic penumbra and substantiated marked metabolism/flow dissociation, which is believed to be a metabolic consequence of the energy demand imposed by repeated peri-infarct depolarizations.
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References
Back T, Zhao W, Ginsberg MD (1995) Three-dimensional image-analysis of brain glucose metabolism/blood flow uncoupling and its electrophysiological correlates in the acute ischemic penumbra following middle cerebral artery occlusion. J Cereb Blood Flow Metab 15: 566–577
Chen Q, Chopp M, Bodzin G, Chen H (1993) Temperature modulation of cerebral depolarization during focal cerebral ischemia in rats: correlation with ischemic injury. J Cereb Blood Flow Metab 13: 389–394
Evans AC, Beil C, Marrett S, Thompson CJ, Hakim A (1988) Anatomical-functional correlation using an adjustable MRIbased region of interest atlas with positron emission tomography. J Cereb Blood Flow Metab 8: 513–530
Ginsberg MD, Pulsinelli WA (1994) Editorial: The ischemic penumbra, injury thresholds, and the therapeutic window for acute stroke. Ann Neurol 36: 553–554
Henderson TC, Triendl EE, Winter R (1985) Edge-and shape-based geometric registration. IEEE Trans Geosci Remote Sensing GE 23: 334–342
Hibbard LS, McGlone JS, Davis DW, Hawkins RA (1987) Three-dimensional representation and analysis of brain energy metabolism. Science 236: 1641–1642
Hossmann K-A (1994) Neurological progess: viability thresholds and the penumbra of focal ischemia. Ann Neurol 36: 557–565
lijima T, Mies G, Hossmann K-A (1992) Repeated negative DC deflections in rat cortex following middle cerebral artery occlusion are abolished by MK-801: effect on volume of ischemic injury. J Cereb Blood Flow Metab 12: 727–733
Maguire GQ Jr, Noz ME, Lee EM, SchimpfJH (1985) Correlation methods for tomographic images using two or three dimensional techniques. Proc Ninth Internat Conf Information Processing in Medical Imaging, Washington, pp 266–279
Markgraf CG, Kraydieh S, Prado R, Watson BD, Dietrich WD, Ginsberg MD (1993) Comparative histopathologic consequences of photothrombotic occlusion of the distal middle cerebral artery in Sprague-Dawley and Wistar rats. Stroke 24: 286–292
Paxinos G, Watson C (1982) The rat brain in stereotaxic coordinates. Academic Press, Sydney
Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with iodo(14C)antipyrine. Am J Physiol 234: H59 - H66
Sokoloff L, Reivich M, Kennedy C, DesRosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M (1977) The (14C)deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anaesthetized albino rat. J Neurochem 28: 897–916
Yao H, Ginsberg MD, Eveleth DD, LaManna JC, Watson BD, Alonso OF, Loor JY, Busto R (1995) Local cerebral glucose utilization and cytoskeletal proteolysis as indices of evolving focal ischemic injury in core and penumbra. J Cereb Blood Flow Metab: 15: 398–408
Young TY, Gunaseekaran S (1988) A regional approach to tracking 3D motion in an image sequence. In: Huang TS (ed) Advances in computer vision and image processing. JAI, USA, pp 63–99
Young TY, Wang YL (1984) Analysis of three dimensional rotation and linear shape changes. Pattern Recognit Lett 2: 239–242
Zhao W, Ginsberg MD, Smith DW (1995) Three-dimensional quantitative autoradiography by disparity analysis: theory and application to image-averaging of local cerebral glucose utilization. J Cereb Blood Flow Metab 15: 552–565
Zhao W, Young TY, Ginsberg MD (1983) Registration and three-dimensional reconstruction of autoradiographic images by the disparity analysis method. IEEE Trans Med Imag 12: 782–791
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© 1996 Springer-Verlag
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Ginsberg, M.D., Back, T., Zhao, W. (1996). Three-Dimensional Metabolic and Hemodynamic Imaging of the Normal and Ischemic Rat Brain. In: Baethmann, A., Kempski, O.S., Plesnila, N., Staub, F. (eds) Mechanisms of Secondary Brain Damage in Cerebral Ischemia and Trauma. Acta Neurochirurgica, vol 66. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9465-2_8
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DOI: https://doi.org/10.1007/978-3-7091-9465-2_8
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