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Brain Edema IX pp 119–120Cite as

Simple Quantitative Evaluation of Blood-Brain Barrier Disruption in Vasogenic Brain Edema

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Part of the book series: Acta Neurochirurgica ((NEUROCHIRURGICA,volume 60))

Summary

Traumatic damage to the brain results in blood-brain barrier disruption with subsequent formation of vasogenic brain edema. The goal of this study was to evaluate a colorimetric assay of Evans blue extravasation for quantitatively studying blood-brain barrier disruption. Vasogenic brain edema was produced by a cortical freezing lesion. 52 male Wistar rats were sacrificed and cardiac perfusion was performed. A volume of dimethylformamide, twice the brain weight, was added to the brain and this was incubated for 72 hours. The supernatant was analyzed spectrophotometrically. Evans blue contents in the lesioned hemisphere was significantly increased within one hour after the lesion production relative to the normal brain and continued to increase for 24 hours (p < 0.01). This quantitative assay of Evans blue is a simple method for evaluation of blood-brain barrier disruption in vasogenic brain edema.

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References

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo 113, Japan

    Yukio Ikeda M.D., D.M.Sc, M. Wang & S. Nakazawa

Authors
  1. Yukio Ikeda M.D., D.M.Sc
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  2. M. Wang
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  3. S. Nakazawa
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Musashino Red-Cross Hospital, Tokyo, Japan

    Umeo Ito M.D. (Director and Neurosurgeon in Chief) (Director and Neurosurgeon in Chief)

  2. Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, München, Federal Republic of Germany

    Alexander Baethmann M.D. (Professor) (Professor)

  3. Max-Planck Institut für Neurologische Forschung, Köln, Federal Republic of Germany

    Konstantin-A. Hossmann M.D. (Professor) (Professor)

  4. Department of Neuropathology, Tokyo Medical and Dental University, Tokyo, Japan

    Toshihiko Kuroiwa M.D. (Associate Professor) (Associate Professor)

  5. Division of Neurosurgery, Medical College of Virginia, Richmond, Virginia, USA

    Anthony Marmarou Ph.D. (Professor) (Professor)

  6. Neurochirurgische Klinik, Ludwig-Maximilians-Universität, München, Federal Republic of Germany

    Hans-J. Reulen M.D. (Professor) (Professor)

  7. Department of Neurosurgery, Tokyo Women’s Medical College, Tokyo, Japan

    Kintomo Takakura M.D. (Professor) (Professor)

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© 1994 Springer-Verlag

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Cite this paper

Ikeda, Y., Wang, M., Nakazawa, S. (1994). Simple Quantitative Evaluation of Blood-Brain Barrier Disruption in Vasogenic Brain Edema. In: Ito, U., et al. Brain Edema IX. Acta Neurochirurgica, vol 60. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9334-1_31

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  • DOI: https://doi.org/10.1007/978-3-7091-9334-1_31

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-9336-5

  • Online ISBN: 978-3-7091-9334-1

  • eBook Packages: Springer Book Archive

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