Summary
We sequenced cDNAs, amplified by the Polymerase Chain Reaction (PCR) which correspond to the carboxy-terminal portion of the 54 KDa protein of various cytopathic (cp) or non-cytopathic (ncp) pestiviral strains. Except for the previously described insertions in two cp strains of the Bovine Viral Diarrhea virus (BVDV), we did not find comparable insertions in this gene. The predicted amino acid sequences of this 54 KDa protein portion contain a conserved cysteine-rich stretch remarkably similar to a “zinc finger”-type binding domain found in many gene-regulatory proteins. Thus, this protein may be involved in the binding to nucleic acids. As the 54 KDa protein is released from its 125 KDa precursor only in the cp strains, we propose the cytopathology may be a consequence of this event.
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© 1991 Springer-Verlag
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De Moerlooze, L., Renard, A., Lecomte, C., Martial, J.A. (1991). A “zinc finger-like” domain in the 54 KDA protein of several pestiviruses. In: Liess, B., Moennig, V., Pohlenz, J., Trautwein, G. (eds) Ruminant Pestivirus Infections. Archives of Virology, vol 3. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9153-8_5
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DOI: https://doi.org/10.1007/978-3-7091-9153-8_5
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