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Cell Organelles pp 3–64Cite as

Organelle Genetics and Transformation of Chlamydomonas

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Part of the book series: Plant Gene Research ((GENE))

Abstract

Of the more than 400 species of unicellular green algae in the genus Chlamydomonas (Ettl, 1976), only a few have been used for genetic investigations. The small cell size, rapid division time, and short sexual cycles of these green flagellates make them especially favorable organisms for genetic and molecular studies. The haploid heterothallic species C. reinhardtii has been the organism of choice for most molecular, cellular and developmental investigations (see Harris, 1989). Its nuclear genome of ca. 1-2 × 105 kb comprises 18 genetic linkage groups (Harris, 1989,1990), and physical maps are being developed for many of these (Ranum et al, 1988). Detailed molecular maps have also been established for the 196 kb chloroplast and 15.8 kb mitochondrial genomes of C. reinhardtii, and formal genetic studies are possible for both organelles (see Harris, 1989,1990). A fourth genome of 6–9 mb, localized in the basal body has recently been defined (Hall et al., 1989) and correlated with a unique quasi-Mendelian circular linkage group (Ramanis and Luck, 1986). Existence of closely related, interfertile isolates such as C. smithii mt + (CC-1373 = SAG 54.72 and UTEX 1062) and the Minnesota S-1 D-2 isolates (CC-2290 and CC-1952) that differ from C. reinhardtii by numerous restriction fragment length polymorphisms (RFLPs) in two or more genomes (Boynton et al, 1987; Gross et al., 1988; Hall et al., 1989; Ranum et al., 1988) makes this species group especially useful for molecular genetic studies.

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Authors and Affiliations

  1. Department of Botany, Duke University, Durham, NC, 27706, USA

    John E. Boynton, Scott M. Newman & Elizabeth H. Harris

  2. Department of Zoology, Duke University, Durham, NC, 27706, USA

    Nicholas W. Gillham

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  3. Scott M. Newman
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  4. Elizabeth H. Harris
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  1. Botanisches Institut, Ludwig-Maximilians-Universität, Munich, Germany

    Reinhold G. Herrmann

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Boynton, J.E., Gillham, N.W., Newman, S.M., Harris, E.H. (1992). Organelle Genetics and Transformation of Chlamydomonas . In: Herrmann, R.G. (eds) Cell Organelles. Plant Gene Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9138-5_1

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