Summary
Potential involvement of neuroexcitatory mechanisms was studied in: 1) repetitive forebrain ischaemia in gerbils, 2) global cerebral ischaemia in rats and 3) cryogenic injury to the cerebral cortex in rats and gerbils. Uptake of 45Ca was used as a marker of injury, whereas ultrastructural localization of calcium was assessed with an oxalate-pyroantimonate method. The blood-brain barrier was evaluated with immunostaining for serum albumin. Changes in extracellular glutamate were estimated by microdialysis and an enzymatic cycling assay. Changes in water content were assessed by specific gravity measurements.
Repetitive ischaemia of 3 × 5 min carotid occlusions produced a cumulative effect with regard to development of oedema and neuronal injury. This was associated with several-fold increments in glutamate release after repeated insults, whereas there was no apparent correlation with energy metabolism disturbances. Other studies revealed in all models a development of secondary foci distant to the primary impact of ischaemia or cold lesions, which were characterized by calcium accumulation in swollen dendrites, chronic neuronal changes and intraneuronal uptake of serum proteins, all of these changes being potentially compatible with involvement of neuroexcitatory mechanisms.
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References
Nitsch C, Klatzo I (1983) Regional patterns of blood-brain barrier breakdown during epileptiform seizures induced by various convulsive agents. J Neurol Sci 59: 305–322
Nitsch C, Goping G, Laursen H, Klatzo I (1986) The blood-brain barrier to horseradish peroxidase at the onset of bicuculline-induced seizures in hypothalamus, pallidum, hippocampus and other selected regions of the rabbit. Acta Neuropath (Berl) 69: 1–16
Nitsch C, Goping G, Klatzo I (1986) Pathophysiological aspects of blood-brain barrier permeability in epileptic seizures. In: Schwarcz R, Ben-Ari Y (eds) Excitatory amino acids and epilepsy. Plenum Publ Corp, New York, pp 175–189
Tomida S, Nowak TS Jr, Vass K, Lohr JM, Klatzo I (1987) Experimental model for repetitive ischaemic attacks in the gerbil: The cumulative effect of repeated ischaemic insults. J Cereb Blood Flow Metab 7: 773–782
Nowak TS Jr, Tomida S, Pluta R, Xu S, Kozuka M, Vass K, Wagner HG, Klatzo I (1990) Cumulative effect of repeated ischaemia on brain oedema in the gerbil. Biochemical and physiological correlates of repeated ischaemia insults. Adv Neurol 52: 1–9
Hossmann K-A, Nagashima G, Klatzo I (1990) Repetitive ischaemia of cat brain: Pathophysiological observations. Neurol Res On press
Nagashima G, Nowak TS Jr, Joó F, Ikeda J, Ruetzler C, Lohr J, Klatzo I (1990) The role of the blood-brain barrier in ischaemic brain lesions. In: Johanssen BB, Owman Ch, Widner H (eds) Pathophysiology of the blood-brain barrier. Elsevier Science Publishers, pp 311–321
Nagashima G, Nowak TS Jr, Joó F, Ikeda J, Ruetzler C, Lohr J, Klatzo I (1990) The role of the blood-brain barrier in ischaemic brain lesions. In: Johanssen BB, Owman Ch, Widner H (eds) Pathophysiology of the blood-brain barrier. Elsevier Science Publishers, pp 311–321
Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic Press, San Diego
Olney JW (1978) Neurotoxicity of excitatory amino acids. In: McGeer EG, Olney JW, McGeer PL (eds) Kainic acid as tool in neurobiology. Raven Press, New York, pp 95–121
Sloviter RS, Dempster DW (1985) “Epileptic” brain damage is replicated qualitatively in the rat hippocampus by central injection of glutamate or aspartate but not by GABA or acetylcholine. Brain Res Bull 15: 39–60
Choi DW (1988) Calcium-mediated neurotoxicity: Relationship to specific channel types and role of ischaemic damage. Trends Neurosci II: 465–469
Benveniste H, Drejer J, Schousboe A, Diemer NH (1984) Elevation of extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischaemia monitored by intracerebral microdialysis. J Neurochem 43: 1369–1374
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© 1990 Springer-Verlag
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Saito, N. et al. (1990). Role of Neuroexcitation in Development of Blood-Brain Barrier and Oedematous Changes Following Cerebral Ischaemia and Traumatic Brain Injury. In: Reulen, HJ., Baethmann, A., Fenstermacher, J., Marmarou, A., Spatz, M. (eds) Brain Edema VIII. Acta Neurochirurgica, vol 51. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9115-6_63
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DOI: https://doi.org/10.1007/978-3-7091-9115-6_63
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